PMID- 10022510 OWN - NLM STAT- MEDLINE DCOM- 19990503 LR - 20191024 IS - 0730-2312 (Print) IS - 0730-2312 (Linking) VI - 72 IP - 2 DP - 1999 Feb 1 TI - Characterization of the human 36-kDa carboxyl terminal LIM domain protein (hCLIM1). PG - 279-85 AB - We characterized a human cDNA clone encoding a 36-kDa carboxyl terminal LIM domain protein with a PDZ domain at the amino terminal. This full-length cDNA clone has a predicted open reading frame (ORF) of 329 amino-acid residues. The ORF of this cDNA encodes the human homolog of rat CLP36, and the putative protein is named human 36-kDa carboxyl terminal LIM domain protein (hCLIM1, nomenclature approved by the HUGO/GDB Nomenclature Committee). The hCLIM1 probe was used to hybridize with poly(A)+ RNA of various human tissues. Strong signals were detected in heart and skeletal muscle; moderate signals were detected in spleen, small intestine, colon, placenta, and lung; weaker levels were detected in liver, thymus, kidney, prostate, and pancreas; and no observable signals were detected in brain, testis, ovary, and peripheral blood leukocytes. The hCLIM1 gene was studied by fluorescence in situ hybridization (FISH), somatic cell hybrid analysis, and radiation hybrid mapping, and it is located at the human chromosome 10q26. FAU - Kotaka, M AU - Kotaka M AD - Department of Biochemistry, Chinese University of Hong Kong, Shatin, NT. FAU - Ngai, S M AU - Ngai SM FAU - Garcia-Barcelo, M AU - Garcia-Barcelo M FAU - Tsui, S K AU - Tsui SK FAU - Fung, K P AU - Fung KP FAU - Lee, C Y AU - Lee CY FAU - Waye, M M AU - Waye MM LA - eng SI - GENBANK/U90878 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Cell Biochem JT - Journal of cellular biochemistry JID - 8205768 RN - 0 (Homeodomain Proteins) RN - 0 (LDB2 protein, human) RN - 0 (LIM Domain Proteins) RN - 0 (Microfilament Proteins) RN - 0 (Transcription Factors) SB - IM MH - Amino Acid Sequence MH - Animals MH - Blotting, Northern MH - Chromosome Mapping MH - Chromosomes, Human, Pair 10/*genetics MH - Gene Library MH - Heart/physiology MH - Homeodomain Proteins/*genetics MH - Humans MH - Hybrid Cells MH - In Situ Hybridization, Fluorescence MH - LIM Domain Proteins MH - Microfilament Proteins/genetics MH - Molecular Sequence Data MH - Open Reading Frames MH - Polymerase Chain Reaction MH - Rats MH - Tissue Distribution MH - Transcription Factors/chemistry/*genetics EDAT- 1999/02/18 03:02 MHDA- 2000/06/20 09:00 CRDT- 1999/02/18 03:02 PHST- 1999/02/18 03:02 [pubmed] PHST- 2000/06/20 09:00 [medline] PHST- 1999/02/18 03:02 [entrez] AID - 10.1002/(SICI)1097-4644(19990201)72:2<279::AID-JCB12>3.0.CO;2-7 [pii] AID - 10.1002/(sici)1097-4644(19990201)72:2<279::aid-jcb12>3.0.co;2-7 [doi] PST - ppublish SO - J Cell Biochem. 1999 Feb 1;72(2):279-85. doi: 10.1002/(sici)1097-4644(19990201)72:2<279::aid-jcb12>3.0.co;2-7.