PMID- 10022580
OWN - NLM
STAT- MEDLINE
DCOM- 19990507
LR  - 20191102
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 38
IP  - 3
DP  - 1999 Feb 15
TI  - Glial cell line-derived neurotrophic factor (GDNF) promotes the survival of 
      axotomized retinal ganglion cells in adult rats: comparison to and combination 
      with brain-derived neurotrophic factor (BDNF).
PG  - 382-90
AB  - Adult rat retinal ganglion cells (RGC) undergo degeneration after optic nerve 
      transection. Studies have shown that exogenously applied neurotrophic factors 
      such as brain-derived neurotrophic factor (BDNF) can attenuate axotomy-induced as 
      well as developmental RGC death. Here, we examined whether glial cell 
      line-derived neurotrophic factor (GDNF), a known neurotrophic factor for 
      dopaminergic neurons and motor neurons, could provide neurotrophic support to RGC 
      in adult rats. We determined whether RGC could retrogradely transport GDNF from 
      their target tissue. After injection into the superior colliculus of adult rats, 
      125I-GDNF was retrogradely transported to contralateral eyes but not to 
      ipsilateral eyes. The transport of 125I-GDNF could be blocked by coinjection of 
      excess unlabeled GDNF, indicating that it was receptor mediated. We tested 
      whether intravitreally applied GDNF could prevent axotomy-induced RGC 
      degeneration. The RGC were prelabeled with Fluorogold (FG) and axotomized by 
      intraorbital optic nerve transection. GDNF, BDNF (positive control), cytochrome c 
      (negative control), or a GDNF/BDNF combination was injected intravitreally on 
      days 0 and 7. On day 14, FG-labeled RGC were counted from whole-mount retinas. We 
      found that, similar to BDNF, GDNF could significantly attenuate the degeneration 
      of RGC in a dose-dependent fashion. Furthermore, the combination treatment of 
      GDNF and BDNF showed better protection than either factor used individually. Our 
      data indicate that GDNF is a neurotrophic factor for the adult rat RGC. GDNF, 
      like BDNF, may be useful for the treatment of human RGC degenerative diseases.
FAU - Yan, Q
AU  - Yan Q
AD  - Department of Neuroscience, Amgen, Inc., Thousand Oaks, California 91320, USA.
FAU - Wang, J
AU  - Wang J
FAU - Matheson, C R
AU  - Matheson CR
FAU - Urich, J L
AU  - Urich JL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GDNF protein, human)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - *Axotomy
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Survival/drug effects
MH  - Female
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Humans
MH  - *Nerve Growth Factors
MH  - Nerve Tissue Proteins/*pharmacology
MH  - Neuroprotective Agents/*pharmacology
MH  - Optic Nerve/physiology
MH  - Rats
MH  - Recombinant Proteins/pharmacology
MH  - Retinal Ganglion Cells/drug effects/*physiology
MH  - Superior Colliculi/cytology/physiology
MH  - Vitreous Body/physiology
EDAT- 1999/02/18 03:02
MHDA- 2000/06/20 09:00
CRDT- 1999/02/18 03:02
PHST- 1999/02/18 03:02 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/02/18 03:02 [entrez]
AID - 10.1002/(SICI)1097-4695(19990215)38:3<382::AID-NEU7>3.0.CO;2-5 [pii]
AID - 10.1002/(sici)1097-4695(19990215)38:3<382::aid-neu7>3.0.co;2-5 [doi]
PST - ppublish
SO  - J Neurobiol. 1999 Feb 15;38(3):382-90. doi: 
      10.1002/(sici)1097-4695(19990215)38:3<382::aid-neu7>3.0.co;2-5.