PMID- 10023067
OWN - NLM
STAT- MEDLINE
DCOM- 19990329
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 439
IP  - 2
DP  - 1999 Feb 19
TI  - Identification of individuals at high risk for head and neck carcinogenesis using 
      chromosome aneuploidy detected by fluorescence in situ hybridization.
PG  - 223-32
AB  - To visualize the accumulation of chromosome abnormalities in head and neck 
      squamous cell carcinomas (HNSCC) and investigate the extension of the abnormal 
      field, we applied the fluorescence in situ hybridization (FISH) technique to 
      tumor cells and cells collected from a large extension of clinically normal 
      buccal mucosa distant from the tumor in 10 patients. DNA probes specific for 14 
      human chromosomes (1, 2, 3, 6, 7, 8, 9, 10, 11, 12, 15, 17, X, and Y) were used 
      in dual-target, dual-color FISH assays. Control specimens were collected from 
      oral mucosa of 10 healthy non-smokers, in order to define the tolerance limits 
      for abnormalities, and from 10 healthy smokers. Extensive aneuploidy was detected 
      in most of tumor specimens, more frequently represented by chromosome gains than 
      losses. Interestingly, the clinically normal distant oral regions displayed 
      chromosomal aneuploidies in seven out of the 10 patients tested. These findings 
      support the occurrence of field cancerization in HNSCC. In addition, interphase 
      FISH is demonstrated as an effective technique for detecting chromosome 
      aneuploidy associated with malignancy and a potential tool for non-invasive 
      screening of individuals at high-risk for HNSCC.
CI  - Copyright 1999 Elsevier Science B.V.
FAU - Ai, H
AU  - Ai H
AD  - Department of Medical Oncology and Colorado Cancer Center, University of Colorado 
      Health Sciences Center, Box B188, Denver, CO 80262, USA.
FAU - Barrera, J E
AU  - Barrera JE
FAU - Pan, Z
AU  - Pan Z
FAU - Meyers, A D
AU  - Meyers AD
FAU - Varella-Garcia, M
AU  - Varella-Garcia M
LA  - eng
GR  - CA46934/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - DNA Probes
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Head and Neck Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Risk Factors
MH  - Smoking/genetics
EDAT- 1999/02/19 00:00
MHDA- 1999/02/19 00:01
CRDT- 1999/02/19 00:00
PHST- 1999/02/19 00:00 [pubmed]
PHST- 1999/02/19 00:01 [medline]
PHST- 1999/02/19 00:00 [entrez]
AID - S1383-5718(98)00199-5 [pii]
AID - 10.1016/s1383-5718(98)00199-5 [doi]
PST - ppublish
SO  - Mutat Res. 1999 Feb 19;439(2):223-32. doi: 10.1016/s1383-5718(98)00199-5.