PMID- 10024327
OWN - NLM
STAT- MEDLINE
DCOM- 19990309
LR  - 20190722
IS  - 0194-911X (Print)
IS  - 0194-911X (Linking)
VI  - 33
IP  - 2
DP  - 1999 Feb
TI  - Genetic and gender influences on sensitivity to focal cerebral ischemia in the 
      stroke-prone spontaneously hypertensive rat.
PG  - 681-5
AB  - We have investigated genetic transmission of increased sensitivity to focal 
      cerebral ischemia and the influence of gender in the stroke-prone spontaneously 
      hypertensive rat (SHRSP). Halothane-anesthetized, 3- to 5-month-old male and 
      female Wistar-Kyoto rats (WKY), SHRSP, and the first filial generation rats (F1 
      crosses 1 and 2) underwent distal (2 mm) permanent middle cerebral artery 
      occlusion (MCAO) by electrocoagulation. Infarct volume was measured by using 
      hematoxylin-eosin-stained sections and image analysis 24 hours after ischemia and 
      expressed as a percentage of the volume of the ipsilateral hemisphere. Infarct 
      volume in males and females grouped together were significantly larger in SHRSP, 
      F1 cross 1 (SHRSP father), and F1 cross 2 (WKY father), at 36.6+/-2.3% 
      (mean+/-SEM, P<0.001, n=15), 25.4+/-2.4% (P<0.01, n=14), and 33. 9+/-1.6% 
      (P<0.001, n=18), respectively, compared with WKY (14+/-2%, n=17). Male F1 cross 1 
      (18.9+/-2.4%, n=6) developed significantly smaller infarcts than male F1 cross 2 
      (32.8+/-2%, n=8, P<0.005). Females, which underwent ischemia during metestrus, 
      developed larger infarcts than respective males. A group of females in which the 
      cycle was not controlled for developed significantly smaller infarcts than 
      females in metestrus. Thus, the increased sensitivity to MCAO in SHRSP is 
      retained in both F1 cross 1 and cross 2 hybrids, suggesting a dominant or 
      codominant trait; response to cerebral ischemia appears to be affected by gender 
      and stage in the estrous cycle. In addition, the male progenitor of the cross 
      (ie, SHRSP versus WKY) influences stroke sensitivity in male F1 cohorts.
FAU - Carswell, H V
AU  - Carswell HV
AD  - Wellcome Surgical Institute, Department of Statistics, University of Glasgow 
      (Scotland, UK). hvola@udcf.gla.ac.uk
FAU - Anderson, N H
AU  - Anderson NH
FAU - Clark, J S
AU  - Clark JS
FAU - Graham, D
AU  - Graham D
FAU - Jeffs, B
AU  - Jeffs B
FAU - Dominiczak, A F
AU  - Dominiczak AF
FAU - Macrae, I M
AU  - Macrae IM
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hypertension
JT  - Hypertension (Dallas, Tex. : 1979)
JID - 7906255
SB  - IM
MH  - Animals
MH  - Brain Ischemia/*genetics/*physiopathology
MH  - Cerebrovascular Disorders/genetics/physiopathology
MH  - Female
MH  - Hypertension/*complications/genetics/physiopathology
MH  - Male
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Sex Factors
EDAT- 1999/02/19 00:00
MHDA- 1999/02/19 00:01
CRDT- 1999/02/19 00:00
PHST- 1999/02/19 00:00 [pubmed]
PHST- 1999/02/19 00:01 [medline]
PHST- 1999/02/19 00:00 [entrez]
AID - 10.1161/01.hyp.33.2.681 [doi]
PST - ppublish
SO  - Hypertension. 1999 Feb;33(2):681-5. doi: 10.1161/01.hyp.33.2.681.