PMID- 10024544
OWN - NLM
STAT- MEDLINE
DCOM- 19990312
LR  - 20200724
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 67
IP  - 3
DP  - 1999 Mar
TI  - Non-serum-dependent chemotactic factors produced by Candida albicans stimulate 
      chemotaxis by binding to the formyl peptide receptor on neutrophils and to an 
      unknown receptor on macrophages.
PG  - 1063-71
AB  - Serum-free culture filtrates of six Candida species and Saccharomyces cerevisiae 
      were found to contain chemoattractants for human polymorphonuclear leukocytes 
      (PMNs) and a mouse macrophage-like cell line, J774. The chemotactic factors 
      differed for the PMN and J774 cells, however, in terms of heat stability, 
      kinetics of liberation by the yeast cells, and divalent cation requirements for 
      production. The chemoattractant in Candida albicans culture filtrates appeared to 
      act through the formyl peptide receptor (FPR) of PMNs, since it was found to 
      induce chemotaxis of Chinese hamster ovary (CHO) cells that were expressing the 
      human FPR but did not induce chemotaxis of wild-type CHO cells. The C. albicans 
      culture filtrates also induced migration of PMNs across confluent monolayers of a 
      human gastrointestinal epithelial cell line, T84; migration occurred in the 
      basolateral-to-apical direction but not the reverse direction, unless the 
      epithelial tight junctions were disrupted. J774 cells did not migrate toward the 
      formylated peptide (fMet-Leu-Phe; fMLF), and chemotaxis toward the C. albicans 
      culture filtrate was not inhibited by an FPR antagonist 
      (t-butoxycarbonyl-Met-Leu-Phe), suggesting that a different receptor mediated 
      J774 cell chemotaxis. In conclusion, we have identified a receptor by which a 
      non-serum-dependent chemotactic factor (NSCF) produced by C. albicans induced 
      chemotaxis of PMNs. Additionally, we have shown that NSCF was active across 
      epithelial monolayers. These findings suggest that NSCFs produced by C. albicans 
      and other yeast species may influence host-pathogen interactions at the 
      gastrointestinal tract mucosal surface by inducing phagocytic-cell infiltration.
FAU - Edens, H A
AU  - Edens HA
AD  - Department of Microbiology, Montana State University-Bozeman, Bozeman, Montana 
      59717, USA. hedens@tex2.oscs.montana.edu
FAU - Parkos, C A
AU  - Parkos CA
FAU - Liang, T W
AU  - Liang TW
FAU - Jesaitis, A J
AU  - Jesaitis AJ
FAU - Cutler, J E
AU  - Cutler JE
FAU - Miettinen, H M
AU  - Miettinen HM
LA  - eng
GR  - AI40108-02/AI/NIAID NIH HHS/United States
GR  - 5T32AW7465/PHS HHS/United States
GR  - AI22735-10/AI/NIAID NIH HHS/United States
GR  - R01 AI022735/AI/NIAID NIH HHS/United States
GR  - R01 HL054229/HL/NHLBI NIH HHS/United States
GR  - R56 AI022735/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Chemotactic Factors)
RN  - 0 (Receptors, Formyl Peptide)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Peptide)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Candida albicans/*immunology
MH  - Cell Line
MH  - Cell Movement
MH  - Chemotactic Factors/*physiology
MH  - *Chemotaxis, Leukocyte
MH  - Cricetinae
MH  - Humans
MH  - Macrophages/*physiology
MH  - Mice
MH  - Neutrophils/*immunology/physiology
MH  - Receptors, Formyl Peptide
MH  - Receptors, Immunologic/*physiology
MH  - Receptors, Peptide/*physiology
PMC - PMC96430
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
PMCR- 1999/03/01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
PHST- 1999/03/01 00:00 [pmc-release]
AID - 0733 [pii]
AID - 10.1128/IAI.67.3.1063-1071.1999 [doi]
PST - ppublish
SO  - Infect Immun. 1999 Mar;67(3):1063-71. doi: 10.1128/IAI.67.3.1063-1071.1999.