PMID- 10025896
OWN - NLM
STAT- MEDLINE
DCOM- 19990304
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 13
IP  - 2
DP  - 1999 Feb
TI  - In vitro infection of CD4+ T lymphocytes with HTLV-I generates immortalized cell 
      lines coexpressing lymphoid and myeloid cell markers.
PG  - 222-9
AB  - The human T cell leukemia/lymphoma virus (HTLV-I) is the etiologic agent of adult 
      T cell leukemia (ATL). CD4+ lymphocytes are the preferential targets of 
      infection, even though other cell types can be infected in vitro by the virus. 
      Although ATL cells show CD3 and CD4 surface markers, some ATL-derived cell lines 
      were reported to express also myeloid antigens. In order to analyze possible 
      phenotypic changes induced by HTLV-I after infection of human lymphocytes, CD4+ 
      cells were isolated from peripheral blood of three healthy donors, by separation 
      through immunomagnetic beads. CD4+ lymphocytes were then infected by coculture 
      with irradiated HTLV-I producing MT-2 cells. The phenotypic profile of infected 
      cells was studied by flow cytometric analysis using monoclonal antibodies against 
      lymphoid (CD3, CD4, TCR alpha/beta) and myelomonocitic markers (CD13, CD14, CD15, 
      CD33, CD34). The results show that HTLV-I immortalized cell lines coexpressed 
      CD13, CD33 and lymphoid markers. No expression of CD14, CD15 and CD34 was 
      observed. These data suggest that the presence of both myeloid and lymphoid 
      phenotype in HTLV-I infected T cells is the results of an induction rather than a 
      selection mechanism.
FAU - Tricarico, M
AU  - Tricarico M
AD  - Institute of Experimental Medicine, National Research Council, Area di Ricerca di 
      Tor Vergata, Rome, Italy.
FAU - Macchi, B
AU  - Macchi B
FAU - D'Atri, S
AU  - D'Atri S
FAU - Morrone, S
AU  - Morrone S
FAU - Bonmassar, E
AU  - Bonmassar E
FAU - Fuggetta, M P
AU  - Fuggetta MP
FAU - Graziani, G
AU  - Graziani G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Biomarkers, Tumor)
SB  - IM
EIN - Leukemia 1999 Jul;13(7):1108
MH  - Biomarkers, Tumor
MH  - Blood Donors
MH  - CD4-Positive T-Lymphocytes/*virology
MH  - Cell Line
MH  - Cell Survival/immunology
MH  - Coculture Techniques
MH  - HTLV-I Infections/*immunology
MH  - Humans
MH  - Immunomagnetic Separation
MH  - Immunophenotyping
MH  - Leukemia, Myeloid/*pathology
MH  - Lymphoma/*pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1038/sj.leu.2401296 [doi]
PST - ppublish
SO  - Leukemia. 1999 Feb;13(2):222-9. doi: 10.1038/sj.leu.2401296.