PMID- 10026117
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 60
IP  - 3
DP  - 1999 Mar
TI  - Cloning, sequencing, and localization of bovine estrogen receptor-beta within the 
      ovarian follicle.
PG  - 691-7
AB  - The potential role of estrogen receptor-beta (ERbeta) in normal ovarian 
      folliculogenesis and in reproductive disorders such as ovarian follicular cysts 
      has not been well defined. Therefore, we were interested in cloning, sequencing, 
      and localizing ERbeta mRNA and protein within the bovine ovary. Bovine ERbeta 
      (bERbeta) was amplified by reverse transcription-polymerase chain reaction 
      (RT-PCR), then cloned and sequenced. Results showed that the open reading frame 
      of bERbeta cDNA spanned 1584 nucleotides encoding a protein of 527 amino acids. 
      The N-terminal region of bERbeta was found to be 80% homologous to human and 
      mouse ERbeta and 79% homologous to rat ERbeta. Bovine ERbeta DNA-binding domain 
      was 100% homologous to human, mouse, and rat ERbeta sequences. The 
      C-terminal/ligand-binding domain of bERbeta was 89% homologous to human, 86% 
      homologous to mouse, and 88% homologous to rat ERbeta. Human and bovine ERbeta 
      amino acid sequences are similar in that their coding region extended farther 5' 
      than initially reported for the published rat ERbeta sequence. Using in situ 
      hybridization and immunohistochemistry, ERbeta mRNA and protein, respectively, 
      were demonstrated to be present in granulosa cells of antral follicles in various 
      stages of follicular growth. These findings suggest a role for bERbeta in ovarian 
      follicular growth and maturation.
FAU - Rosenfeld, C S
AU  - Rosenfeld CS
AD  - Departments of Animal Sciences, University of Missouri at Columbia, MO 65211, 
      USA.
FAU - Yuan, X
AU  - Yuan X
FAU - Manikkam, M
AU  - Manikkam M
FAU - Calder, M D
AU  - Calder MD
FAU - Garverick, H A
AU  - Garverick HA
FAU - Lubahn, D B
AU  - Lubahn DB
LA  - eng
SI  - GENBANK/AF110402
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - *Cattle
MH  - *Cloning, Molecular
MH  - DNA/metabolism
MH  - DNA, Complementary/analysis/chemistry
MH  - Female
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Ovarian Follicle/*chemistry
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptors, Estrogen/*analysis/chemistry/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1095/biolreprod60.3.691 [doi]
PST - ppublish
SO  - Biol Reprod. 1999 Mar;60(3):691-7. doi: 10.1095/biolreprod60.3.691.