PMID- 10026125
OWN - NLM
STAT- MEDLINE
DCOM- 19990311
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 60
IP  - 3
DP  - 1999 Mar
TI  - Luteal regression in the normally cycling rat: apoptosis, monocyte 
      chemoattractant protein-1, and inflammatory cell involvement.
PG  - 740-6
AB  - In hypophysectomized rats, prolactin induces regression of the corpora lutea. 
      Luteal regression is accompanied by infiltration of monocytes/macrophages, 
      declines in luteal mass and plasma progestins, and increased staining for 
      monocyte chemoattractant protein-1 (MCP-1). We investigated whether similar 
      events are induced during the estrous cycle, after the proestrous prolactin 
      surge. Rats were killed on proestrus or on estrus, and one ovary was frozen for 
      immunohistochemical detection of MCP-1, monocytes/macrophages (ED1-positive), and 
      differentiated macrophages (ED2-positive) and for in situ detection of apoptotic 
      nuclei. Corpora lutea of the current (proestrus) or preceding (estrus) cycle were 
      dissected from the ovaries of additional rats and frozen for the same analyses 
      and for determination of total protein content. In sections of whole ovaries, 
      intensity and distribution of MCP-1 staining were increased in corpora lutea of 
      multiple ages on estrus as compared to proestrus, as were numbers of 
      differentiated macrophages and apoptotic nuclei per high-power field. Sections of 
      isolated corpora lutea showed these increases on estrus, and the number of 
      monocytes/macrophages per high-power field was also significantly increased. 
      Accompanying these inflammatory/immune events, the corpora lutea on estrus showed 
      decreased weight and total protein per corpus luteum, as compared to corpora 
      lutea on proestrus. These changes are consistent with a proposed role for 
      prolactin in the initiation of luteal apoptosis and of a sequence of 
      inflammatory/immune events that accompany regression of the rat corpus luteum 
      during the normal estrous cycle.
FAU - Bowen, J M
AU  - Bowen JM
AD  - Department of Physiology, University of Michigan, Ann Arbor 48109, USA.
FAU - Towns, R
AU  - Towns R
FAU - Warren, J S
AU  - Warren JS
FAU - Landis Keyes, P
AU  - Landis Keyes P
LA  - eng
GR  - HD-33478/HD/NICHD NIH HHS/United States
GR  - P30 HD-18258/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Cell Count
MH  - Chemokine CCL2/analysis/*physiology
MH  - Corpus Luteum/chemistry
MH  - Estrus
MH  - Female
MH  - Immunohistochemistry
MH  - Luteolysis/*physiology
MH  - Macrophages/cytology/*physiology
MH  - Monocytes/cytology/*physiology
MH  - Proestrus
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1999/02/20 00:00
MHDA- 1999/02/20 00:01
CRDT- 1999/02/20 00:00
PHST- 1999/02/20 00:00 [pubmed]
PHST- 1999/02/20 00:01 [medline]
PHST- 1999/02/20 00:00 [entrez]
AID - 10.1095/biolreprod60.3.740 [doi]
PST - ppublish
SO  - Biol Reprod. 1999 Mar;60(3):740-6. doi: 10.1095/biolreprod60.3.740.