PMID- 10027645
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 721
IP  - 1
DP  - 1999 Jan 8
TI  - Determination of non-protein bound phenylbutazone in bovine plasma using 
      ultrafiltration and liquid chromatography with ultraviolet detection.
PG  - 141-5
AB  - A liquid chromatographic procedure using UV detection was coupled with 
      ultrafiltration for the quantitation of free phenylbutazone in bovine plasma, in 
      the range of 20 ng/ml to 2.0 microg/ml. Whole plasma samples (0.5 to 1 ml) were 
      placed in a 2-ml centrifugal concentrator with a molecular-mass cut-off membrane 
      of 10000 and centrifuged at 4500 g for 2 h at 4 degrees C using a fixed angle 
      rotor. The ultrafiltrate was transferred to an LC vial with a 200-microl insert 
      and 100 microl was injected into an LC system. The chromatographic system used a 
      C18 reversed-phase column connected to a UV detector set at 264 nm. The mobile 
      phase was 0.2 M sodium phosphate buffer (pH 7)-methanol (1:1). Recoveries of 
      phenylbutazone from protein-free plasma water fortified at levels of 20 ng/ml to 
      2 microg/ml ranged from 91 to 93%, with relative standard deviations (R.S.D.s) 
      ranging from 1 to 4%. The concentration of incurred non-protein bound 
      phenylbutazone obtained from a cow intravenously dosed twice with 2 g 
      phenylbutazone, 8 h apart, was 111, 26 and 11 ng/ml for 2, 72 and 104 h post 
      first phenylbutazone dose, respectively.
FAU - De Veau, E J
AU  - De Veau EJ
AD  - U.S. Food and Drug Administration, Center for Veterinary Medicine, Laurel, MD 
      20708, USA. edeveau@bangate.fda.gov
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - GN5P7K3T8S (Phenylbutazone)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*blood
MH  - Cattle
MH  - Chromatography, Liquid/*methods
MH  - Phenylbutazone/*blood
MH  - Spectrophotometry, Ultraviolet
MH  - Ultrafiltration/*methods
EDAT- 1999/02/23 00:00
MHDA- 1999/02/23 00:01
CRDT- 1999/02/23 00:00
PHST- 1999/02/23 00:00 [pubmed]
PHST- 1999/02/23 00:01 [medline]
PHST- 1999/02/23 00:00 [entrez]
AID - 10.1016/s0378-4347(98)00487-3 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1999 Jan 8;721(1):141-5. doi: 
      10.1016/s0378-4347(98)00487-3.