PMID- 10028170
OWN - NLM
STAT- MEDLINE
DCOM- 19990430
LR  - 20200407
IS  - 0378-1135 (Print)
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 64
IP  - 2-3
DP  - 1999 Jan
TI  - Detection and genotyping of bovine diarrhea virus by reverse 
      transcription-polymerase chain amplification of the 5' untranslated region.
PG  - 155-67
AB  - A reverse-transcription polymerase chain reaction (RT-PCR) was developed to 
      differentiate the bovine diarrhea virus (BVDV) from other pestiviruses, and to 
      determine the genotype of the BVDV isolates. For this purpose, primer pairs were 
      selected in the 5' untranslated region (5'UTR). The primers BE and B2 were 
      located in highly conserved regions and were pestivirus-specific. Two primer 
      pairs named B3B4 and B5B6 were specific of BVDV genotypes I and II, respectively. 
      With this technique, an amplification product of the expected size was obtained 
      with either the B3B4 or the B5B6 primer pairs for the 107 BVDV isolates tested 
      but not for BDV or CSFV. For some isolates that were grouped in the genotype II, 
      sequence analysis of the PCR fragments confirmed their classification into this 
      genotype.
FAU - Letellier, C
AU  - Letellier C
AD  - Centre d'Etude et de Recherches Veterinaires et Agrochimiques 99, Brussels, 
      Belgium. calet@var.fgov.be
FAU - Kerkhofs, P
AU  - Kerkhofs P
FAU - Wellemans, G
AU  - Wellemans G
FAU - Vanopdenbosch, E
AU  - Vanopdenbosch E
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 5' Untranslated Regions/*chemistry
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern/veterinary
MH  - Bovine Virus Diarrhea-Mucosal Disease/*diagnosis
MH  - Cattle
MH  - DNA Primers/chemistry
MH  - Diarrhea Viruses, Bovine Viral/classification/*genetics/isolation & purification
MH  - Electrophoresis, Agar Gel
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Viral/chemistry
MH  - Reverse Transcriptase Polymerase Chain Reaction/*veterinary
MH  - Sequence Alignment
PMC - PMC7117503
EDAT- 1999/02/24 00:00
MHDA- 1999/02/24 00:01
PMCR- 1999/01/29
CRDT- 1999/02/24 00:00
PHST- 1999/02/24 00:00 [pubmed]
PHST- 1999/02/24 00:01 [medline]
PHST- 1999/02/24 00:00 [entrez]
PHST- 1999/01/29 00:00 [pmc-release]
AID - S0378-1135(98)00267-3 [pii]
AID - 10.1016/s0378-1135(98)00267-3 [doi]
PST - ppublish
SO  - Vet Microbiol. 1999 Jan;64(2-3):155-67. doi: 10.1016/s0378-1135(98)00267-3.