PMID- 10029234
OWN - NLM
STAT- MEDLINE
DCOM- 19990504
LR  - 20131121
IS  - 0887-4476 (Print)
IS  - 0887-4476 (Linking)
VI  - 31
IP  - 3
DP  - 1999 Mar 1
TI  - N-tert-butyl-alpha-phenylnitrone protects against 
      3,4-methylenedioxymethamphetamine-induced depletion of serotonin in rats.
PG  - 169-77
AB  - The present study examined the effect of N-tert-butyl-alpha-phenylnitrone (PBN) 
      on 3,4-methylenedioxmathamphetamine (MDMA)-induced depletion of serotonin in the 
      CNS. Rats were treated with two concurrent injections of MDMA (20 mg/kg, s.c.), 
      PBN (50-400 mg/kg dissolved in ethanol, 50 mg/ml of 25% ethanol, i.p.), saline or 
      25% ethanol, alone or in combination, 6 h apart, and sacrificed 5 days later. 
      Rectal temperature was measured prior to and hourly following the drug injection 
      for 5 h. Monoamine levels in the tissue were measured by HPLC. Density of the 
      5-HT transporters was assayed by [3H]paroxetine binding. Rectal temperature of 
      rats increased after MDMA, decreased after PBN, ethanol, PBN plus ethanol, and 
      MDMA plus ethanol, and was not significantly altered after MDMA plus PBN. Levels 
      of 5-HT and 5-HIAA in the frontal cortex, hippocampus, striatum, and brain stem 
      of rats decreased significantly after MDMA or MDMA plus ethanol, but not after 
      MDMA plus PBN, PBN plus ethanol (PBN dissolved in ethanol), or ethanol as 
      compared to the saline controls. Levels of 5-HT and 5-HIAA in the brain tissues 
      of rats treated with MDMA plus PBN were elevated as compared to those treated 
      with MDMA plus saline. Similar results were observed in the density of 5-HT 
      transporters in the frontal cortex and hippocampus. These results indicate that 
      scavenging of free radicals of MDMA metabolites or reactive oxygen species by PBN 
      and with lowering of body temperature protected against MDMA-induced depletion of 
      serotonin transmitter.
FAU - Yeh, S Y
AU  - Yeh SY
AD  - Molecular Neuropsychiatry Section, National Institute on Drug Abuse, Intramural 
      Research Program, National Institutes of Health, Baltimore, Maryland 21224, USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Synapse
JT  - Synapse (New York, N.Y.)
JID - 8806914
RN  - 0 (Cyclic N-Oxides)
RN  - 0 (Free Radicals)
RN  - 0 (Nitrogen Oxides)
RN  - 0 (Serotonin Agents)
RN  - 0 (Spin Labels)
RN  - 333DO1RDJY (Serotonin)
RN  - 3I91332OPG (phenyl-N-tert-butylnitrone)
RN  - 54-16-0 (Hydroxyindoleacetic Acid)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Body Temperature Regulation/drug effects
MH  - Brain/cytology/*drug effects/metabolism
MH  - Brain Stem/drug effects/metabolism
MH  - Cyclic N-Oxides
MH  - Free Radicals
MH  - Frontal Lobe/drug effects/metabolism
MH  - Hippocampus/drug effects/metabolism
MH  - Hydroxyindoleacetic Acid/metabolism
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*antagonists & inhibitors
MH  - Neurons/drug effects/metabolism
MH  - Nitrogen Oxides/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serotonin/*deficiency
MH  - Serotonin Agents/*pharmacology
MH  - Spin Labels
EDAT- 1999/02/24 03:02
MHDA- 2000/06/20 09:00
CRDT- 1999/02/24 03:02
PHST- 1999/02/24 03:02 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/02/24 03:02 [entrez]
AID - 10.1002/(SICI)1098-2396(19990301)31:3<169::AID-SYN1>3.0.CO;2-N [pii]
AID - 10.1002/(SICI)1098-2396(19990301)31:3<169::AID-SYN1>3.0.CO;2-N [doi]
PST - ppublish
SO  - Synapse. 1999 Mar 1;31(3):169-77. doi: 
      10.1002/(SICI)1098-2396(19990301)31:3<169::AID-SYN1>3.0.CO;2-N.