PMID- 10029451
OWN - NLM
STAT- MEDLINE
DCOM- 19990302
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 30
IP  - 2
DP  - 1999 Feb
TI  - Expression of proopiomelanocortin (POMC)-derived melanocyte-stimulating hormone 
      (MSH) and adrenocorticotropic hormone (ACTH) peptides in skin of basal cell 
      carcinoma patients.
PG  - 208-15
AB  - We proposed that local expression and production of proopiomelanocortin (POMC) 
      peptides may play a role in human skin physiology and pathology, including the 
      development and progression of skin cancers. Reverse transcription polymerase 
      chain reaction (RT-PCR) and Northern blotting hybridization techniques were used 
      to study gene expression. Reversed-phase (RP) high-pressure liquid chromatography 
      (HPLC) separation with subsequent radioimmunoassays were used to identify 
      alpha-melanocyte-stimulating hormone (alpha-MSH) and adrenocorticotropic hormone 
      (ACTH) peptides. Immunocytochemistry (IHC) was used to localize ACTH, alpha-MSH, 
      and beta-MSH antigens in skin. RT-PCR, RP-HPLC, and IHC analyses documented the 
      expression of POMC mRNA and production of ACTH and alpha-MSH peptides in lesional 
      and perilesional skin of basal cell carcinoma (BCC) patients and in cultured 
      keratinocytes, which was accompanied by the expression of the MC1-R gene encoding 
      the receptor activated by MSH and ACTH. Thirty specimens were analyzed by IHC. 
      Immunoreactive alpha-MSH, beta-MSH, and ACTH were detected, in 21 of 21, in 11 of 
      20, and in 6 of 8 of lesional skin, and in 6 of 6, in 5 of 7, and in 6 of 8 
      perilesional skin specimens analyzed, respectively. Antigen distribution was 
      heterogenous and present in BCC, epidermis, hair follicles, dermal mononuclear 
      cells, and extracellular matrix. We conclude that messenger RNA for POMC, MC1-R, 
      and the peptides MSH and ACTH are produced in skin of BCC patients. Because 
      keratinocytes are a target for MSH and ACTH bioregulation, the production of 
      these peptides is stimulated by UVB, and the peptides can act as 
      immunosupressors, we suggest that MSH and ACTH may facilitate development of BCC.
FAU - Slominski, A
AU  - Slominski A
AD  - Department of Pathology, Loyola University Medical Center, Maywood, IL 60153, 
      USA.
FAU - Heasley, D
AU  - Heasley D
FAU - Mazurkiewicz, J E
AU  - Mazurkiewicz JE
FAU - Ermak, G
AU  - Ermak G
FAU - Baker, J
AU  - Baker J
FAU - Carlson, J A
AU  - Carlson JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Corticotropin)
RN  - 0 (Receptors, Melanocortin)
RN  - 66796-54-1 (Pro-Opiomelanocortin)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - 9002-79-3 (Melanocyte-Stimulating Hormones)
SB  - IM
MH  - Adrenocorticotropic Hormone/*biosynthesis/genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, Neoplasm/metabolism
MH  - Carcinoma, Basal Cell/*metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Melanocyte-Stimulating Hormones/*biosynthesis/genetics
MH  - Middle Aged
MH  - Pro-Opiomelanocortin/biosynthesis/genetics
MH  - RNA, Messenger/metabolism
MH  - Receptors, Corticotropin/metabolism
MH  - Receptors, Melanocortin
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 1999/02/24 00:00
MHDA- 1999/02/24 00:01
CRDT- 1999/02/24 00:00
PHST- 1999/02/24 00:00 [pubmed]
PHST- 1999/02/24 00:01 [medline]
PHST- 1999/02/24 00:00 [entrez]
AID - S0046-8177(99)90278-2 [pii]
AID - 10.1016/s0046-8177(99)90278-2 [doi]
PST - ppublish
SO  - Hum Pathol. 1999 Feb;30(2):208-15. doi: 10.1016/s0046-8177(99)90278-2.