PMID- 10029467
OWN - NLM
STAT- MEDLINE
DCOM- 19990223
LR  - 20170214
IS  - 0022-0345 (Print)
IS  - 0022-0345 (Linking)
VI  - 78
IP  - 2
DP  - 1999 Feb
TI  - Production of matrix-degrading enzymes and inhibition of osteoclast-like cell 
      differentiation by fibroblast-like cells from the periodontal ligament of human 
      primary teeth.
PG  - 681-9
AB  - Clinically, the most apparent difference between the primary and permanent 
      dentitions is the physiologic loss of the primary tooth by root resorption. Root 
      resorption is associated with loss of integrity of the periodontal ligament 
      (PDL), followed by recruitment of resorptive cells that remove root structure. We 
      therefore cultured primary dentition PDL fibroblasts (PPDL cells) to investigate 
      in vitro their production of matrix metalloproteinases (MMPs) and tissue 
      inhibitors of MMP (TIMPs), and the effects of soluble factors produced by these 
      cells on osteoclast-like cell differentiation. These studies demonstrate that 
      PPDL cells in vitro have a heterogeneous morphology, and they constitutively 
      synthesize 92-kDa gelatinase, 72-kDa gelatinase, and 53/57-kDa procollagenase as 
      well as TIMP-1, -2, and a third inhibitor of matrix metalloproteinase, as 
      determined by substrate gel zymography and immunoblot analysis. Compared with PDL 
      cells from the permanent dentition, PPDL cells generally produced a greater 
      amount of collagenase but similar amounts of the gelatinases and inhibitors. PPDL 
      cells were treated with pro-inflammatory cytokines to determine their effect on 
      the expression of matrix-degrading enzymes and inhibitors. Interleukin-1alpha and 
      tumor necrosis factor-alpha enhanced the constitutive expression of proteinases 
      but not that of inhibitors in PPDL cells. Conditioned media from PPDL cell lines 
      inhibited the differentiation of osteoclast-like cells in mouse bone marrow 
      cultures. These findings indicate that PPDL cells may modulate the cascade of 
      root resorption both by their regulated production of proteinases and inhibitors 
      and by synthesis of unknown soluble factor(s) that may regulate osteoclast 
      development.
FAU - Wu, Y M
AU  - Wu YM
AD  - Department of Growth and Development, School of Dentistry, University of 
      California-San Francisco, 94143-0754, USA.
FAU - Richards, D W
AU  - Richards DW
FAU - Rowe, D J
AU  - Rowe DJ
LA  - eng
GR  - R03 DE-11183/DE/NIDCR NIH HHS/United States
GR  - R29 DE-10062/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Interleukin-1)
RN  - 0 (Protease Inhibitors)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
MH  - Adult
MH  - Analysis of Variance
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Line
MH  - Child, Preschool
MH  - Culture Media, Conditioned
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme Induction/drug effects
MH  - Extracellular Matrix/enzymology
MH  - Female
MH  - Fibroblasts/enzymology
MH  - Humans
MH  - Immunoblotting
MH  - Interleukin-1/pharmacology
MH  - Male
MH  - Metalloendopeptidases/antagonists & inhibitors/*biosynthesis
MH  - Mice
MH  - Osteoclasts/enzymology
MH  - Periodontal Ligament/cytology/*enzymology
MH  - Protease Inhibitors/metabolism
MH  - Root Resorption/*enzymology
MH  - Tissue Inhibitor of Metalloproteinases/biosynthesis
MH  - Tooth, Deciduous/*enzymology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1999/02/24 00:00
MHDA- 1999/02/24 00:01
CRDT- 1999/02/24 00:00
PHST- 1999/02/24 00:00 [pubmed]
PHST- 1999/02/24 00:01 [medline]
PHST- 1999/02/24 00:00 [entrez]
AID - 10.1177/00220345990780020801 [doi]
PST - ppublish
SO  - J Dent Res. 1999 Feb;78(2):681-9. doi: 10.1177/00220345990780020801.