PMID- 10029851
OWN - NLM
STAT- MEDLINE
DCOM- 19990517
LR  - 20031114
IS  - 0003-1488 (Print)
IS  - 0003-1488 (Linking)
VI  - 214
IP  - 4
DP  - 1999 Feb 15
TI  - Evaluation of serologic and viral detection methods for diagnosing feline 
      herpesvirus-1 infection in cats with acute respiratory tract or chronic ocular 
      disease.
PG  - 502-7
AB  - OBJECTIVE: To determine the value of virus isolation (VI), immunofluorescent 
      antibody (IFA) assay, serum neutralization (SN), and ELISA for the diagnosis of 
      clinical feline herpesvirus-1 (FHV-1) infection in cats. ANIMALS: 46 clinically 
      normal cats, 17 cats with signs of acute respiratory tract disease, and 38 cats 
      with signs of chronic ocular disease. PROCEDURE: Conjunctival swabs for VI, 
      conjunctival scrapings for IFA testing, and venous blood samples for SN or ELISA 
      testing were obtained from all cats. RESULTS: FHV-1 was detected in 10.9 and 
      28.3% of clinically normal cats and in 18.2 and 33.3% of cats with 
      FHV-1-associated disease by VI and the IFA assay, respectively. There were no 
      significant differences in the viral detection rate between cats with acute 
      respiratory tract disease and cats with chronic ocular disease or between 
      diseased cats and clinically normal cats; however, FHV-1 was never detected by 
      both methods in clinically normal cats. Overall FHV-1 seroprevalence was 97% when 
      tested by ELISA and 66% when tested by SN. Seroprevalence did not vary 
      significantly among the 3 groups for either serologic test. Magnitude of SN and 
      ELISA titers varied greatly but independently of presence or absence of clinical 
      signs of FHV-1-associated disease. Sensitivity, specificity, and positive and 
      negative predictive values were assessed for VI and the IFA assay--jointly and 
      individually--and for each SN and ELISA titer magnitude. Values never all 
      exceeded 50%. CLINICAL IMPLICATIONS: Because FHV-1 can be detected commonly in 
      clinically normal cats by the IFA assay or VI, neither test appears to aid in the 
      clinical diagnosis of FHV-1 infection. Seroprevalence does not appear to vary 
      between affected and clinically normal cats. SN, ELISA, VI, and the IFA assay 
      appear to be of limited value in the diagnosis of FHV-1-associated disease in 
      cats. Concurrent assessment of the IFA assay and VI results may permit exclusion 
      of FHV-1 as an etiologic agent if results of both tests are negative.
FAU - Maggs, D J
AU  - Maggs DJ
AD  - Department of Clinical Sciences, College of Veterinary Medicine and Biomedical 
      Sciences, Colorado State University, Fort Collins 80523, USA.
FAU - Lappin, M R
AU  - Lappin MR
FAU - Reif, J S
AU  - Reif JS
FAU - Collins, J K
AU  - Collins JK
FAU - Carman, J
AU  - Carman J
FAU - Dawson, D A
AU  - Dawson DA
FAU - Bruns, C
AU  - Bruns C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Am Vet Med Assoc
JT  - Journal of the American Veterinary Medical Association
JID - 7503067
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Cat Diseases/*diagnosis/epidemiology/virology
MH  - Cats
MH  - Cell Line
MH  - Chronic Disease
MH  - Conjunctiva/virology
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Eye Infections, Viral/diagnosis/*veterinary/virology
MH  - Female
MH  - Fluorescent Antibody Technique/veterinary
MH  - Herpesviridae/immunology/*isolation & purification
MH  - Herpesviridae Infections/diagnosis/epidemiology/*veterinary
MH  - Male
MH  - Neutralization Tests/veterinary
MH  - Respiratory Tract Infections/diagnosis/*veterinary/virology
MH  - Sensitivity and Specificity
MH  - Seroepidemiologic Studies
EDAT- 1999/02/25 00:00
MHDA- 1999/02/25 00:01
CRDT- 1999/02/25 00:00
PHST- 1999/02/25 00:00 [pubmed]
PHST- 1999/02/25 00:01 [medline]
PHST- 1999/02/25 00:00 [entrez]
PST - ppublish
SO  - J Am Vet Med Assoc. 1999 Feb 15;214(4):502-7.