PMID- 10036278
OWN - NLM
STAT- MEDLINE
DCOM- 19990817
LR  - 20171213
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 81
IP  - 2
DP  - 1999 Feb
TI  - Depression of synaptic efficacy in high- and low-output Drosophila neuromuscular 
      junctions by the molting hormone (20-HE).
PG  - 788-94
AB  - The molt-related steroid hormone, 20-hydroxyecdysone (20-HE), was applied to 
      muscles 6 and 7 of third instar larval of Drosophila melanogaster neuromuscular 
      junction preparations to examine if rapid, nongenomic responses could be observed 
      as was shown recently to occur in crustacean neuromuscular junctions. At a dose 
      of 10 microM, the excitatory junction potentials were reduced in amplitude within 
      minutes. To elucidate the site of action of the hormone, focal-macropatch 
      recordings of synaptic currents were obtained over the neuromuscular junctions. 
      The results showed that the high-output (Is) and the low-output (Ib) motor nerve 
      terminals, which innervate muscles 6 and 7, released fewer synaptic vesicles for 
      each stimulation while exposed to 20-HE. Because the size and shape of synaptic 
      currents from spontaneous releases did not change, the effects of the 20-HE are 
      presynaptic. The rapid effects of this hormone may account in part for the 
      quiescent behavior associated with molts among insects and crustaceans.
FAU - Ruffner, M E
AU  - Ruffner ME
AD  - Thomas Hunt Morgan School of Biological Sciences, Nerve-Muscle Group, University 
      of Kentucky, Lexington, Kentucky 40506-0225, USA.
FAU - Cromarty, S I
AU  - Cromarty SI
FAU - Cooper, R L
AU  - Cooper RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 5289-74-7 (Ecdysterone)
SB  - IM
MH  - Animals
MH  - Drosophila melanogaster
MH  - Ecdysterone/*pharmacology
MH  - Excitatory Postsynaptic Potentials/drug effects
MH  - In Vitro Techniques
MH  - Larva/physiology
MH  - Motor Neurons/drug effects/physiology
MH  - Muscles/innervation
MH  - Neuromuscular Junction/*drug effects/physiology
MH  - Patch-Clamp Techniques
MH  - Synaptic Transmission/*drug effects/physiology
MH  - Synaptic Vesicles/drug effects
MH  - Time Factors
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
AID - 10.1152/jn.1999.81.2.788 [doi]
PST - ppublish
SO  - J Neurophysiol. 1999 Feb;81(2):788-94. doi: 10.1152/jn.1999.81.2.788.