PMID- 10037326
OWN - NLM
STAT- MEDLINE
DCOM- 19990304
LR  - 20161124
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 33
IP  - 1
DP  - 1999
TI  - Base-substitution profiles of externally activated polycyclic aromatic 
      hydrocarbons and aromatic amines determined in a lacZ reversion assay.
PG  - 75-85
AB  - Using an improved set of lactose-auxotrophic Escherichia coli tester strains, the 
      proportion of the six possible transitions and transversions after mutagen 
      exposure was assessed. Mutagenic specificity was determined in 
      plate-incorporation assays using lactose-containing minimal medium for the 
      selection of revertants, either after application of directly acting mutagens or 
      by including a metabolic activation system with rat liver S9-extract. The 
      differential and dose-dependent response of the six tester strains was shown by 
      treating the bacteria with described diagnostic mutagens and other directly DNA 
      damaging substances, e.g., N-methyl-N-nitrosoguanidine (MNNG) and 
      benzo[a]pyrene-diolepoxide (BPDE). Polycyclic aromatic hydrocarbons and aromatic 
      amines were investigated in the presence of an external metabolic activation 
      system. Benzo[a]pyrene (BaP) yielded similar mutation profiles as its ultimate 
      mutagen BPDE, if 100-fold increased doses were applied. In contrast to the 
      mutation profile of BaP, which was dominated by G:C-T:A transversions, 
      mutagenesis with benzo[c]phenanthrene (BcPh) produced predominantly A:T-T:A 
      transversions. The same base change was observed with 5-methylchrysene and found 
      to be missing with 5,6-dimethylchrysene, while both compounds caused G:C-A:T 
      transitions. The aromatic amines 4-aminobiphenyl (4-ABP), 2-aminoanthracene 
      (2-AA) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhlP) yielded similar 
      yet distinguishable mutation profiles. Base-substitution reversion profiles of 
      the chemical mutagens were in agreement with those obtained in other systems and 
      with molecular analysis of mutants induced by these agents.
FAU - Garganta, F
AU  - Garganta F
AD  - Analytisch-biologisches Forschungslabor, Munchen, Germany.
FAU - Krause, G
AU  - Krause G
FAU - Scherer, G
AU  - Scherer G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 0 (Amines)
RN  - 0 (Aminobiphenyl Compounds)
RN  - 0 (Anthracenes)
RN  - 0 (Imidazoles)
RN  - 0 (Liver Extracts)
RN  - 0 (Mutagens)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 16054949HJ (4-biphenylamine)
RN  - 8240818JGU (2-anthramine)
RN  - 909C6UN66T (2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine)
SB  - IM
MH  - Amines/*pharmacokinetics/toxicity
MH  - Aminobiphenyl Compounds/toxicity
MH  - Anthracenes/toxicity
MH  - Biotransformation
MH  - Dose-Response Relationship, Drug
MH  - Escherichia coli/drug effects/genetics/growth & development
MH  - Imidazoles/toxicity
MH  - *Lac Operon/drug effects
MH  - Liver Extracts/toxicity
MH  - Mutagenicity Tests/methods
MH  - Mutagens/toxicity
MH  - *Point Mutation
MH  - Polycyclic Aromatic Hydrocarbons/*pharmacokinetics/toxicity
MH  - Salmonella typhimurium/drug effects/genetics/growth & development
EDAT- 1999/02/26 00:00
MHDA- 1999/02/26 00:01
CRDT- 1999/02/26 00:00
PHST- 1999/02/26 00:00 [pubmed]
PHST- 1999/02/26 00:01 [medline]
PHST- 1999/02/26 00:00 [entrez]
AID - 10.1002/(SICI)1098-2280(1999)33:1<75::AID-EM9>3.0.CO;2-1 [pii]
PST - ppublish
SO  - Environ Mol Mutagen. 1999;33(1):75-85.