PMID- 10049044
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 13
IP  - 1
DP  - 1999 Jan
TI  - Frequent monoallelic loss of D13S319 in multiple myeloma patients shown by 
      interphase fluorescence in situ hybridization.
PG  - 105-9
AB  - Deletions or monosomy of chromosome 13 are frequent in multiple myeloma (MM). A 
      candidate tumor suppressor gene might reside telomeric of the retinoblastoma gene 
      (RBl) at band 13q14 and to play a role in B cell neoplasm. The D13S319 locus, 
      between RB1 and D13S25 loci at 13q14 is the most commonly deleted marker in 
      chronic lymphocytic leukemia (CLL) and non-Hodgkin's lymphoma (NHL). We evaluated 
      the D13S319 locus in 24 MM cases by fluorescence in situ hybridization (FISH). We 
      observed monosomy for D13S319 in 6/20 (30%) MM patients with an apparently normal 
      karyotype. As expected, in four karyotypically abnormal MM cases with partial or 
      complete monosomy for chromosome 13, all of them had monoallelic loss of D13S319. 
      Our results indicated that the loss of D13S319 is commonly found in MM, even at 
      diagnosis, and is more frequent than predicted based on conventional cytogenetic 
      analysis of metaphase spreads. This finding implicates a candidate tumor 
      suppressor gene at 13q14 in the pathogenesis of MM.
FAU - Chang, H
AU  - Chang H
AD  - Hematology Division, The Toronto Hospital, Ontario, Canada.
FAU - Bouman, D
AU  - Bouman D
FAU - Boerkoel, C F
AU  - Boerkoel CF
FAU - Stewart, A K
AU  - Stewart AK
FAU - Squire, J A
AU  - Squire JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow/pathology
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 13
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interphase
MH  - Karyotyping
MH  - *Loss of Heterozygosity
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics/pathology
EDAT- 1999/02/12 00:00
MHDA- 1999/02/12 00:01
CRDT- 1999/02/12 00:00
PHST- 1999/02/12 00:00 [pubmed]
PHST- 1999/02/12 00:01 [medline]
PHST- 1999/02/12 00:00 [entrez]
AID - 10.1038/sj.leu.2401208 [doi]
PST - ppublish
SO  - Leukemia. 1999 Jan;13(1):105-9. doi: 10.1038/sj.leu.2401208.