PMID- 10049046
OWN - NLM
STAT- MEDLINE
DCOM- 19990225
LR  - 20200328
IS  - 0887-6924 (Print)
IS  - 1476-5551 (Electronic)
IS  - 0887-6924 (Linking)
VI  - 13
IP  - 1
DP  - 1999 Jan
TI  - Identification of 9-O acetyl sialoglycoconjugates (9-OAcSGs) as biomarkers in 
      childhood acute lymphoblastic leukemia using a lectin, AchatininH, as a probe.
PG  - 119-25
AB  - Neoplastic transformation causes changes in cell surface architecture, most 
      notably, aberrant sialylation. Exploiting the restricted specificity of a 9-O 
      acetyl sialic acid (9-OAcSA) binding lectin, AchatininH (ATN(H)), we have 
      identified two 9-O acetyl sialoglyconjugates (9-OAcSGs) on lymphoblasts of 87 
      children suffering from acute lymphoblastic leukemia (ALL). The preferential 
      binding of ATN(H) to lymphoblasts induces their 11-fold increased agglutination 
      (81 +/- 7.8%) compared to peripheral blood mononuclear cells (PBMC) of normal 
      donors (8 +/- 4.3%) which corroborates with flow cytometry studies. Agglutination 
      of MOLT-4 (87 +/- 4.8%), a lymphoblastoid cell line and MDCK (91.25 +/- 0.01%), a 
      cell line expressing surface 9-OAcSA, confirms the preferential binding of ATN(H) 
      to lymphoblasts through their surface 9-OAcSGs. Furthermore, fluorometric 
      quantitation reveals a 4.6-fold increase in % of 9-OAcSA on lymphoblasts of ALL 
      patients (42.1 +/- 4.1%) compared to normal donors (9.2 +/- 3.4%). Western 
      blotting confirms that ATN(H) recognizes two membrane sialoglycoconjugates, of MW 
      120 kDa and 90 kDa, both having 9-OAcSA alpha2 --> 6 GalNAc terminal sugar moiety 
      as their lectinogenic epitope. We propose that these 9-OAcSGs may serve as 
      biomarkers for detection and monitoring of lymphoblasts in ALL and accordingly 
      merit therapeutic considerations.
FAU - Sinha, D
AU  - Sinha D
AD  - Indian Institute of Chemical Biology, Calcutta.
FAU - Mandal, C
AU  - Mandal C
FAU - Bhattacharya, D K
AU  - Bhattacharya DK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Disaccharides)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Lectins)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Trisaccharides)
RN  - 0 (achatinin(H))
SB  - IM
MH  - Agglutination Tests/methods
MH  - Animals
MH  - Biomarkers, Tumor/*blood
MH  - Carbohydrate Sequence
MH  - Child
MH  - Disaccharides
MH  - Flow Cytometry/methods
MH  - Humans
MH  - Indicators and Reagents
MH  - *Lectins
MH  - Lymphocytes/cytology/*pathology
MH  - Molecular Sequence Data
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/blood/*diagnosis
MH  - Reference Values
MH  - Sensitivity and Specificity
MH  - Sialoglycoproteins/*blood
MH  - Snails
MH  - Trisaccharides
MH  - Tumor Cells, Cultured
PMC - PMC7100560
EDAT- 1999/02/12 00:00
MHDA- 1999/02/12 00:01
PMCR- 2020/03/27
CRDT- 1999/02/12 00:00
PHST- 1999/02/12 00:00 [pubmed]
PHST- 1999/02/12 00:01 [medline]
PHST- 1999/02/12 00:00 [entrez]
PHST- 2020/03/27 00:00 [pmc-release]
AID - BF2401239 [pii]
AID - 10.1038/sj.leu.2401239 [doi]
PST - ppublish
SO  - Leukemia. 1999 Jan;13(1):119-25. doi: 10.1038/sj.leu.2401239.