PMID- 10049761
OWN - NLM
STAT- MEDLINE
DCOM- 19990401
LR  - 20181201
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 255
IP  - 3
DP  - 1999 Feb 24
TI  - Differential regulation of ligand-dependent and ligand-independent functions of 
      the mouse retinoid X receptor beta by alternative splicing.
PG  - 625-30
AB  - RXR is involved in two distinct signal transduction pathways. RXR activates 
      transcription ligand-dependently on RXRE and behaves as a positive heterodimer 
      partner with permissive receptors; while it behaves as a ligand-independent 
      silent partner with nonpermissive receptors. We studied the functions of a unique 
      isoform of mouse RXRbeta2 (mRXRbeta2E) which has an in-frame four-amino-acid 
      insertion in the ligand binding domain. mRXRbeta2E did not bind 9-cis RA with 
      high affinity and did not activate transcription on RXRE. However, mRXRbeta2E 
      formed heterodimers with TR, VDR, RAR, and LXR, and enhanced transcriptional 
      activity of the nonpermissive VDR to the same extent as mRXRbeta2. With the 
      permissive LXR, mRXRbeta2E did not show 9-cis RA-dependent transactivation and 
      inhibited mRXRbeta2 activity. Thus, although mRXRbeta2E can perform 
      ligand-independent functions of RXR, it cannot mediate the ligand-dependent 
      functions of RXR, indicating that alternative splicing plays an important role in 
      the differential regulation of these two important functions of RXR.
CI  - Copyright 1999 Academic Press.
FAU - Fujita, A
AU  - Fujita A
AD  - The Third Department of Internal Medicine, Faculty of Medicine, University of 
      Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
FAU - Mitsuhashi, T
AU  - Mitsuhashi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
RN  - 1UA8E65KDZ (Alitretinoin)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Alitretinoin
MH  - Alternative Splicing/*genetics
MH  - Animals
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/genetics
MH  - Gene Expression Regulation/genetics
MH  - *Ligands
MH  - Mice
MH  - Protein Binding
MH  - Receptors, Retinoic Acid/*genetics
MH  - Regulatory Sequences, Nucleic Acid/genetics
MH  - Retinoid X Receptors
MH  - Sequence Analysis, DNA
MH  - Signal Transduction
MH  - Spodoptera/genetics
MH  - Transcription Factors/*genetics
MH  - Transcriptional Activation/genetics
MH  - Tretinoin/metabolism
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - S0006-291X(99)90243-7 [pii]
AID - 10.1006/bbrc.1999.0243 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Feb 24;255(3):625-30. doi: 
      10.1006/bbrc.1999.0243.