PMID- 10049950 OWN - NLM STAT- MEDLINE DCOM- 19990602 LR - 20190508 IS - 0022-1007 (Print) IS - 1540-9538 (Electronic) IS - 0022-1007 (Linking) VI - 189 IP - 5 DP - 1999 Mar 1 TI - Activated human T cells, B cells, and monocytes produce brain-derived neurotrophic factor in vitro and in inflammatory brain lesions: a neuroprotective role of inflammation? PG - 865-70 AB - Brain-derived neurotrophic factor (BDNF) has potent effects on neuronal survival and plasticity during development and after injury. In the nervous system, neurons are considered the major cellular source of BDNF. We demonstrate here that in addition, activated human T cells, B cells, and monocytes secrete bioactive BDNF in vitro. Notably, in T helper (Th)1- and Th2-type CD4(+) T cell lines specific for myelin autoantigens such as myelin basic protein or myelin oligodendrocyte glycoprotein, BDNF production is increased upon antigen stimulation. The BDNF secreted by immune cells is bioactive, as it supports neuronal survival in vitro. Using anti-BDNF monoclonal antibody and polyclonal antiserum, BDNF immunoreactivity is demonstrable in inflammatory infiltrates in the brain of patients with acute disseminated encephalitis and multiple sclerosis. The results raise the possibility that in the nervous system, inflammatory infiltrates have a neuroprotective effect, which may limit the success of nonselective immunotherapies. FAU - Kerschensteiner, M AU - Kerschensteiner M AD - Department of Neuroimmunology, Max Planck Institute for Neurobiology, D-82152 Martinsried, Germany. FAU - Gallmeier, E AU - Gallmeier E FAU - Behrens, L AU - Behrens L FAU - Leal, V V AU - Leal VV FAU - Misgeld, T AU - Misgeld T FAU - Klinkert, W E AU - Klinkert WE FAU - Kolbeck, R AU - Kolbeck R FAU - Hoppe, E AU - Hoppe E FAU - Oropeza-Wekerle, R L AU - Oropeza-Wekerle RL FAU - Bartke, I AU - Bartke I FAU - Stadelmann, C AU - Stadelmann C FAU - Lassmann, H AU - Lassmann H FAU - Wekerle, H AU - Wekerle H FAU - Hohlfeld, R AU - Hohlfeld R LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Exp Med JT - The Journal of experimental medicine JID - 2985109R RN - 0 (Autoantigens) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Glycoproteins) RN - 0 (Myelin Basic Protein) RN - 0 (RNA, Messenger) SB - IM MH - Autoantigens/immunology MH - B-Lymphocytes/*immunology MH - Brain Diseases/*immunology MH - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics MH - Encephalitis/immunology MH - Glycoproteins/immunology MH - Humans MH - Inflammation/immunology MH - Lymphocyte Activation MH - Monocytes/*immunology MH - Multiple Sclerosis/immunology MH - Myelin Basic Protein/immunology MH - Neurodegenerative Diseases/immunology MH - Oligodendroglia/immunology MH - RNA, Messenger/isolation & purification MH - T-Lymphocytes/*immunology MH - Transcription, Genetic PMC - PMC2192942 EDAT- 1999/03/02 00:00 MHDA- 1999/03/02 00:01 PMCR- 1999/09/01 CRDT- 1999/03/02 00:00 PHST- 1999/03/02 00:00 [pubmed] PHST- 1999/03/02 00:01 [medline] PHST- 1999/03/02 00:00 [entrez] PHST- 1999/09/01 00:00 [pmc-release] AID - 10.1084/jem.189.5.865 [doi] PST - ppublish SO - J Exp Med. 1999 Mar 1;189(5):865-70. doi: 10.1084/jem.189.5.865.