PMID- 10050017
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20190513
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 515 ( Pt 2)
IP  - Pt 2
DP  - 1999 Mar 1
TI  - Involvement of protein kinase C in 5-HT-stimulated ciliary activity in Helisoma 
      trivolvis embryos.
PG  - 511-22
AB  - 1. During development, embryos of the pulmonate gastropod, Helisoma trivolvis, 
      undergo a rotation behaviour due to the co-ordinated beating of three bands of 
      ciliated epithelial cells. This behaviour is in part mediated by the 
      neurotransmitter serotonin (5-HT) released from a pair of identified embryonic 
      neurons. Using time-lapse videomicroscopy to measure ciliary beat frequency (CBF) 
      in response to pharmacological manipulations, we determined whether protein 
      kinase C (PKC) is involved in mediating 5-HT-stimulated ciliary beating. 2. 
      Diacylglycerol (DAG) analogues sn-1,2-dioctanoyl glycerol (DiC8; 100 microM) and 
      1-oleoyl-2-acetyl-sn-glycerol (OAG; 100 microM), partially mimicked the 
      5-HT-induced increase in CBF. In contrast, application of OAG in the absence of 
      extracellular Ca2+ did not result in an increase in CBF. 3. 5-HT-stimulated CBF 
      was effectively blocked by PKC inhibitors bisindolylmaleimide (10 and 100 nM) and 
      calphostin C (10 nM). In addition, bisindolylmaleimide (100 nM) inhibited 
      DiC8-induced increases in CBF. At a higher concentration (200 nM), 
      bisindolylmaleimide did not significantly reduce 5-HT-stimulated 
      cilio-excitation. 4. Two different phorbol esters, phorbol 12-myristate 
      13-acetate (TPA; 0.1, 10 or 1000 nM) and phorbol 12beta, 13alpha-dibenzoate 
      (PDBn; 10 microM) did not alter basal CBF. TPA (1 microM) did not alter 
      5-HT-stimulated CBF. Likewise, the synthetic form of phosphatidylserine, 
      N-(6-phenylhexyl)-5-chloro-1-naphthalenesulphonamide (SC-9; 10 microM), did not 
      increase CBF, whereas a strong increase in CBF was observed upon exposure to 
      5-HT. 5. The results suggest that a DAG-dependent, phorbol ester-insensitive 
      isoform of PKC mediates 5-HT-stimulated CBF in ciliated epithelial cells from 
      embryos of Helisoma trivolvis.
FAU - Christopher, K J
AU  - Christopher KJ
AD  - Department of Biological Sciences, CW-405 Biological Sciences Building, 
      University of Alberta, Edmonton, Alberta, Canada T6G 2E9.
FAU - Young, K G
AU  - Young KG
FAU - Chang, J P
AU  - Chang JP
FAU - Goldberg, J I
AU  - Goldberg JI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Diglycerides)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Phorbol Esters)
RN  - 25405-85-0 (phorbol-12,13-dibenzoate)
RN  - 333DO1RDJY (Serotonin)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Cilia/*drug effects/*physiology
MH  - Diglycerides/pharmacology
MH  - Embryo, Nonmammalian/drug effects/physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Phorbol Esters/pharmacology
MH  - Protein Kinase C/antagonists & inhibitors/*physiology
MH  - Serotonin/*pharmacology
MH  - Snails/embryology
MH  - Tetradecanoylphorbol Acetate/pharmacology
PMC - PMC2269170
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
PMCR- 2000/03/01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
PHST- 2000/03/01 00:00 [pmc-release]
AID - 10.1111/j.1469-7793.1999.511ac.x [doi]
PST - ppublish
SO  - J Physiol. 1999 Mar 1;515 ( Pt 2)(Pt 2):511-22. doi: 
      10.1111/j.1469-7793.1999.511ac.x.