PMID- 10050719
OWN - NLM
STAT- MEDLINE
DCOM- 19990330
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 104
IP  - 2
DP  - 1999 Feb
TI  - Expression of interleukin-1beta and tumour necrosis factor-alpha in plasma cells 
      from patients with multiple myeloma.
PG  - 350-7
AB  - Interleukin-1beta(IL-1beta) and tumour necrosis factor-alpha (TNF-alpha) are 
      potent bone resorbing cytokines that may contribute to the development of the 
      osteolytic bone disease observed in patients with multiple myeloma (MM). Although 
      these factors have been identified in cultures of bone marrow mononuclear cells 
      isolated from patients, the identity of the cells responsible for producing 
      IL-1beta and TNFalpha remains unclear. Using a sensitive dual-colour fluorescence 
      in situ hybridization (FISH) technique and a two-colour immunofluorescence method 
      we have investigated the expression of the mRNA and protein, for IL-1beta and 
      TNFalpha, by individual bone marrow plasma cells from patients with MM and 
      monoclonal gammopathy of undetermined significance (MGUS). The mRNA for IL-1beta 
      and TNFalpha was identified in all cells expressing the immunoglobulin light 
      chain from all patients with MM and MGUS. However, the IL-1beta protein could not 
      be detected in cytoplasmic light chain positive cells in any of the patients 
      examined. In contrast, the TNFalpha protein was detected in clonal plasma cells 
      from patients with both MM and MGUS. Interestingly, the IL-1beta and TNFalpha 
      mRNA and proteins were readily detected within a small proportion of the 
      non-plasma cells from patients with both MM and MGUS. These data suggest that 
      myeloma cells in vivo are able to produce TNFalpha but not IL-1beta. In addition, 
      a small proportion of accessory cells are likely to be able to contribute to the 
      production of both ILbeta and TNFalpha.
FAU - Sati, H I
AU  - Sati HI
AD  - Division of Biochemical and Musculoskeletal Medicine, University of Sheffield 
      Medical School.
FAU - Greaves, M
AU  - Greaves M
FAU - Apperley, J F
AU  - Apperley JF
FAU - Russell, R G
AU  - Russell RG
FAU - Croucher, P I
AU  - Croucher PI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Interleukin-1/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/metabolism
MH  - Paraproteinemias/*metabolism
MH  - Plasma Cells/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 1999/03/02 00:00
MHDA- 1999/03/02 00:01
CRDT- 1999/03/02 00:00
PHST- 1999/03/02 00:00 [pubmed]
PHST- 1999/03/02 00:01 [medline]
PHST- 1999/03/02 00:00 [entrez]
AID - 10.1046/j.1365-2141.1999.01193.x [doi]
PST - ppublish
SO  - Br J Haematol. 1999 Feb;104(2):350-7. doi: 10.1046/j.1365-2141.1999.01193.x.