PMID- 10051711
OWN - NLM
STAT- MEDLINE
DCOM- 19990421
LR  - 20201209
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 29
IP  - 1
DP  - 1999 Jan
TI  - Comparison of cytokine secretion profiles provoked in mice by glutaraldehyde and 
      formaldehyde.
PG  - 124-32
AB  - BACKGROUND: Glutaraldehyde and formaldehyde are commonly used sterilizing agents 
      that are known skin sensitizers. There is some controversy, however, regarding 
      their capacity to cause respiratory allergy. The authors have demonstrated 
      previously that topical exposure of mice to chemical contact allergens such as 
      2,4-dinitrochlorobenzene (DNCB) or respiratory allergens such as trimellitic 
      anhydride (TMA) induces characteristic cytokine secretion profiles consistent 
      with the selective activation of T helper1 (TH1)- and TH2-type cells, 
      respectively. OBJECTIVE: To investigate the quality of immune response provoked 
      following topical exposure of mice to these materials. METHODS: BALB/c strain 
      mice were exposed topically to 50% formaldehyde or to various concentrations of 
      glutaraldehyde in acetone. Control animals were treated concurrently with the 
      reference contact allergen DNCB (1% in acetone:olive oil [AOO]) or with the 
      reference respiratory sensitizer TMA (10% in AOO). Thirteen days after the 
      initiation of exposure, draining lymph node cells (LNCs) were cultured for 
      12-120h and cytokine content of supernatants analysed by cytokine-specific 
      enzyme-linked immunosorbent assay. RESULTS: DNCB-alpha and formaldehyde-activated 
      LNCs produced high levels of the TH1-type cytokine interferon gamma, but little 
      of the TH2-type products interleukins 4 and 10. TMA- and 
      glutaraldehyde-stimulated LNCs displayed the converse TH2-type pattern of 
      cytokine expression. CONCLUSIONS: These data are consistent with glutaraldehyde, 
      but not formaldehyde, having significant potential to cause allergic 
      sensitization of the respiratory tract.
FAU - Dearman, R J
AU  - Dearman RJ
AD  - Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, UK.
FAU - Basketter, D A
AU  - Basketter DA
FAU - Evans, P
AU  - Evans P
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Disinfectants)
RN  - 0 (Irritants)
RN  - 0 (Phthalic Anhydrides)
RN  - 130068-27-8 (Interleukin-10)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 207137-56-2 (Interleukin-4)
RN  - 80T61EUU7H (trimellitic anhydride)
RN  - 82115-62-6 (Interferon-gamma)
RN  - T3C89M417N (Glutaral)
SB  - IM
MH  - Administration, Topical
MH  - Allergens/pharmacology
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cytokines/*drug effects/metabolism
MH  - Dinitrochlorobenzene/pharmacology
MH  - Disinfectants/*pharmacology
MH  - Formaldehyde/*pharmacology
MH  - Glutaral/*pharmacology
MH  - Interferon-gamma/drug effects/metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-4/metabolism
MH  - Irritants/pharmacology
MH  - Lymph Nodes/cytology/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phthalic Anhydrides/pharmacology
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
AID - 10.1046/j.1365-2222.1999.00437.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 1999 Jan;29(1):124-32. doi: 10.1046/j.1365-2222.1999.00437.x.