PMID- 10052690
OWN - NLM
STAT- MEDLINE
DCOM- 19990611
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 721
IP  - 2
DP  - 1999 Jan 22
TI  - Sensitive high-performance liquid chromatographic determination with fluorescence 
      detection of phenol and chlorophenols with 
      4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride as a labeling reagent.
PG  - 179-86
AB  - A sensitive HPLC method for the determination of phenol and chlorophenols was 
      developed. The fluorescence labeling reaction of phenols with 
      4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) was completed in 30 
      min at 60 degrees C. The separation of DIB-derivatives of five representative 
      phenols, i.e., phenol, o-, p-chlorophenol, 2,4-dichlorophenol, 
      2,4,6-trichlorophenol, was achieved within 35 min with an ODS column using 
      isocratic elution. The detection limits of these DIB derivatives at a 
      signal-to-noise ratio (SIN) of 3 were in the range of 0.024 to 0.08 microM 
      (0.12-0.45 pmol/20 microl injection). Twelve kinds of DIB derivatives with 
      phenols containing mono-, di-, tri-, tetra- and penta-chlorophenol were also well 
      separated within 208 min by changing the elution conditions. The derivatives were 
      stable for at least for 24 h when they were placed at room temperature in the 
      dark. The proposed method was applied to the assay of human urine samples and 
      free and total phenol were determined. The relative standard deviations (RSDs) of 
      the proposed method for within and between-day assay were <7.0% and <14.2%, 
      respectively. The average concentrations of free and total phenol found in urine 
      (n=6) were 4.3+/-2.5 and 29.5+/-14.0 microM, respectively.
FAU - Wada, M
AU  - Wada M
AD  - School of Pharmaceutical Sciences, Nagasaki University, Japan.
FAU - Kinoshita, S
AU  - Kinoshita S
FAU - Itayama, Y
AU  - Itayama Y
FAU - Kuroda, N
AU  - Kuroda N
FAU - Nakashima, K
AU  - Nakashima K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride)
RN  - 0 (Benzoates)
RN  - 0 (Chlorophenols)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Imidazoles)
RN  - 0 (Phenols)
SB  - IM
MH  - Benzoates
MH  - Calibration
MH  - Chlorophenols/*urine
MH  - Chromatography, High Pressure Liquid
MH  - Fluorescent Dyes
MH  - Humans
MH  - Imidazoles
MH  - Phenols/*urine
MH  - Spectrometry, Fluorescence
EDAT- 1999/03/03 00:00
MHDA- 1999/03/03 00:01
CRDT- 1999/03/03 00:00
PHST- 1999/03/03 00:00 [pubmed]
PHST- 1999/03/03 00:01 [medline]
PHST- 1999/03/03 00:00 [entrez]
AID - 10.1016/s0378-4347(98)00499-x [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1999 Jan 22;721(2):179-86. doi: 
      10.1016/s0378-4347(98)00499-x.