PMID- 10063233
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20191102
IS  - 1341-8076 (Print)
IS  - 1341-8076 (Linking)
VI  - 24
IP  - 6
DP  - 1998 Dec
TI  - Detection of numerical chromosome abnormalities in human spermatozoa by 
      three-color fluorescence in situ hybridization.
PG  - 385-92
AB  - Three-color fluorescence in situ hybridization (FISH) was used to detect 
      numerical X, Y, and 17 chromosomal aberrations in human sperm nuclei. 
      Digoxigeninlabeled alpha satellite chromosome X-specific probe DXZ1, 
      biotin-labeled classical satellite chromosome Y-specific probe DYZ1, and biotin 
      plus digoxigenin-labeled alpha satellite chromosome 17-specific probe D17Z1 were 
      simultaneously hybridized to sperm preparations from donors with normal semen 
      (group A) and abnormal semen (group B) characteristics. The proportions of 
      haploid X, Y, 17 and disomy, diploidy of them before and after swim-up were 
      determined. At least 3,000 sperm were analyzed for each sample. Overall, up to 
      98% of sperm were labeled with the probes, and all statistical analyses were 
      performed using chi 2 tests. A significant difference was observed between group 
      A and group B in frequency of sex chromosome disomy (p < 0.05). In group B, there 
      were significant differences in frequencies of sex chromosomes disomy (p < 0.05) 
      and diploidy (p < 0.01) before to after swim-up. There was no significant 
      difference in frequency of disomy 17 between the 2 groups. In group A and B, the 
      ratios of X- to Y-bearing sperm were 1:1 (neat semen), but in both groups there 
      was a significant increase in Y-bearing sperm after swim-up. The results of this 
      study demonstrated that abnormal semen has sex chromosome disomy more frequently 
      than does normal semen and that portion of sex chromosome disomic and diploid 
      sperm is removed by swim-up, especially for abnormal semen. These findings 
      suggest that we should be careful in using abnormal semen for IVF, especially for 
      ICSI, and should perform swim-up if possible.
FAU - Li, P
AU  - Li P
AD  - Department of Obstetrics and Gynaecology, Kinki University School of Medicine, 
      Osaka, Japan.
FAU - Hoshiai, H
AU  - Hoshiai H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - J Obstet Gynaecol Res
JT  - The journal of obstetrics and gynaecology research
JID - 9612761
SB  - IM
MH  - *Chromosome Aberrations
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infertility, Male/*genetics
MH  - Male
MH  - *Spermatozoa
EDAT- 1999/03/04 00:00
MHDA- 1999/03/04 00:01
CRDT- 1999/03/04 00:00
PHST- 1999/03/04 00:00 [pubmed]
PHST- 1999/03/04 00:01 [medline]
PHST- 1999/03/04 00:00 [entrez]
AID - 10.1111/j.1447-0756.1998.tb00113.x [doi]
PST - ppublish
SO  - J Obstet Gynaecol Res. 1998 Dec;24(6):385-92. doi: 
      10.1111/j.1447-0756.1998.tb00113.x.