PMID- 10063534
OWN - NLM
STAT- MEDLINE
DCOM- 19990520
LR  - 20220330
IS  - 0378-1135 (Print)
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 64
IP  - 4
DP  - 1999 Feb 12
TI  - Porcine reproductive and respiratory syndrome virus and Haemophilus parasuis 
      antigen distribution in dually infected pigs.
PG  - 287-97
AB  - Immunohistochemical, viral and bacterial isolation techniques were used to study 
      the distribution and localization of porcine reproductive and respiratory 
      syndrome virus (PRRSV) and Haemophilus (H.) parasuis in experimentally infected 
      pigs. Thirty pigs seronegative to PRRSV and H. parasuis were divided into four 
      groups. Group A pigs (10 animals) were inoculated with both virus and bacteria; 
      group B pigs (10 animals) were inoculated with bacteria, group C pigs (five 
      animals) were inoculated with virus and group D pigs (five animals) were kept as 
      negative controls. All pigs of groups A and C became infected with PRRSV, 
      according to virological techniques used (immunohistochemistry, virus isolation 
      and virus serology). Lung, heart and tonsils were the most frequently 
      immunolabeled tissues, and monocyte/macrophage lineage cells were the target for 
      PRRSV in all tissues. All pigs in groups A and B also became infected with H. 
      parasuis based on immunohistochemical and bacterial isolation results. Serosal 
      surfaces, lung and tonsils were the most frequently immunolabeled tissues, and 
      bacteria were found in monocyte/macrophage lineage cells as well as within 
      neutrophil cytoplasm. No differences in terms of bacterial distribution or 
      localization in tissues of pigs of groups A and B were detected. These results 
      suggest that there is no influence of the previous infection with PRRSV in the 
      occurrence of H. parasuis infection.
FAU - Segales, J
AU  - Segales J
AD  - U.D. Anatomia Patologica, Facultat de Veterinaria, Universitat Autonoma de 
      Barcelona, Spain. qsegales@cc.uab.es
FAU - Domingo, M
AU  - Domingo M
FAU - Solano, G I
AU  - Solano GI
FAU - Pijoan, C
AU  - Pijoan C
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antigens, Bacterial/analysis
MH  - Antigens, Viral/analysis
MH  - Fluorescent Antibody Technique, Indirect/veterinary
MH  - Haemophilus/*immunology/isolation & purification
MH  - Haemophilus Infections/complications/microbiology/*veterinary
MH  - Histocytochemistry
MH  - Lung/cytology/immunology
MH  - Myocardium/cytology/immunology
MH  - Palatine Tonsil/cytology/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/isolation & 
      purification
MH  - Swine
MH  - *Swine Diseases/microbiology/virology
MH  - Viremia/veterinary
PMC - PMC7117397
EDAT- 1999/03/04 00:00
MHDA- 1999/03/04 00:01
PMCR- 1999/01/29
CRDT- 1999/03/04 00:00
PHST- 1999/03/04 00:00 [pubmed]
PHST- 1999/03/04 00:01 [medline]
PHST- 1999/03/04 00:00 [entrez]
PHST- 1999/01/29 00:00 [pmc-release]
AID - S0378-1135(98)00260-0 [pii]
AID - 10.1016/s0378-1135(98)00260-0 [doi]
PST - ppublish
SO  - Vet Microbiol. 1999 Feb 12;64(4):287-97. doi: 10.1016/s0378-1135(98)00260-0.