PMID- 10063608
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190831
IS  - 0301-4622 (Print)
IS  - 0301-4622 (Linking)
VI  - 76
IP  - 2
DP  - 1999 Feb 1
TI  - Gangliosides asymmetrically alter the membrane order in cultured PC-12 cells.
PG  - 117-32
AB  - Exogenous gangliosides readily associate with the cell membranes and produce 
      marked effects on cell growth and differentiation. We have studied the effect of 
      bovine brain gangliosides (BBG) on the membrane dynamics of intact cells. The 
      structural and dynamic changes in the cell membrane were monitored by the 
      fluorescence probes DPH, TMA-DPH and laurdan. Incorporation of BBG into the cell 
      membrane decreased the fluorescence intensity, lifetime and the steady state 
      anisotropy of TMA-DPH. Analysis of the time resolved anisotropy decay by wobbling 
      in the cone model revealed that BBG decreased the order parameter, and increased 
      the cone angle without altering the rotational relaxation rate. The fluorescence 
      intensity and lifetime of DPH were unaffected by BBG incorporation, however, a 
      modest increase was observed in the steady state anisotropy. BBG incorporation 
      reduced the total fluorescence intensity of laurdan with pronounced quenching of 
      the 440-nm band. The wavelength sensitivity of generalized polarization of 
      laurdan manifested an ordered liquid crystalline environment of the probe in the 
      cell membrane. BBG incorporation reduced the GP values and augmented the liquid 
      crystalline behavior of the cell membrane. BBG incorporation also influenced the 
      permeability of cell membranes to cations. An influx of Na+ and Ca2+ and an 
      efflux of K+ was observed. The data demonstrate that incorporation of 
      gangliosides into the cell membrane substantially enhances the disorder and 
      hydration of the lipid bilayer region near the exoplasmic surface. The inner core 
      region near the center of the bilayer becomes slightly more ordered and remains 
      highly hydrophobic. Such changes in the structure and dynamics of the membrane 
      could play an important role in modulation of transmembrane signaling events by 
      the gangliosides.
FAU - Ravichandra, B
AU  - Ravichandra B
AD  - Department of Biophysics, National Institute of Mental Health and Neurosciences, 
      Bangalore, India.
FAU - Joshi, P G
AU  - Joshi PG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biophys Chem
JT  - Biophysical chemistry
JID - 0403171
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Gangliosides)
RN  - 0 (Laurates)
RN  - 0 (Membranes, Artificial)
RN  - CKR7XL41N4 (2-Naphthylamine)
RN  - Y97FBL93VW (laurdan)
SB  - IM
MH  - 2-Naphthylamine/analogs & derivatives/metabolism
MH  - Animals
MH  - Anisotropy
MH  - Brain Chemistry
MH  - Cattle
MH  - Cell Membrane/chemistry/*drug effects
MH  - Cell Survival/physiology
MH  - Fluorescent Dyes
MH  - Gangliosides/*pharmacology
MH  - Laurates/metabolism
MH  - Membranes, Artificial
MH  - Microscopy, Fluorescence
MH  - PC12 Cells
MH  - Rats
EDAT- 1999/03/04 00:00
MHDA- 1999/03/04 00:01
CRDT- 1999/03/04 00:00
PHST- 1999/03/04 00:00 [pubmed]
PHST- 1999/03/04 00:01 [medline]
PHST- 1999/03/04 00:00 [entrez]
AID - S0301-4622(98)00222-1 [pii]
AID - 10.1016/s0301-4622(98)00222-1 [doi]
PST - ppublish
SO  - Biophys Chem. 1999 Feb 1;76(2):117-32. doi: 10.1016/s0301-4622(98)00222-1.