PMID- 10063718
OWN - NLM
STAT- MEDLINE
DCOM- 19990316
LR  - 20161124
IS  - 0578-1337 (Print)
IS  - 0578-1337 (Linking)
VI  - 62
IP  - 2
DP  - 1999 Feb
TI  - Detection of PAX3-FKHR and PAX7-FKHR fusion transcripts in rhabdomyosarcoma by 
      reverse transcriptase-polymerase chain reaction using paraffin-embedded tissue.
PG  - 86-91
AB  - BACKGROUND: Alveolar rhabdomyosarcoma (RMS) is associated with a characteristic 
      chromosomal translocation t(2;13)(q35;q14). The genes involved in this 
      translocation are paired box (PAX)3 on chromosome 2 and forkhead in RMS (FKHR) on 
      chromosome 13. An occasional variant translocation t(1;13)(p36;q14) affecting 
      PAX7 and FKHR on chromosomes 1 and 13, respectively, has also been described. 
      Chromosomal translocations in RMS are detected using conventional cytogenetic 
      analysis, fluorescence in situ hybridization (FISH) or reverse 
      transcriptase-polymerase chain reaction (RT-PCR) on fresh or frozen tissue 
      samples. We describe the results of RT-PCR analysis of PAX3-FKHR and PAX7-FKHR 
      chimeric messages in formalin-fixed, paraffin-embedded tissue samples from 17 RMS 
      cases. METHODS: RNA was extracted from formalin-fixed, paraffin-embedded RMS 
      tissue. Oligonucleotide primers corresponding to the regions of PAX3, PAX7 and 
      FKHR were used for the detection of PAX3-FKHR and PAX7-FKHR chimeric messages. A 
      seminested PCR of the PCR products was used to increase the sensitivity of 
      detection. The amplified fragments were purified and directly sequenced to 
      confirm the specificity of the methods. RESULTS: The PAX3-FKHR chimeric message 
      was detected in all three cases of alveolar RMS but not in any of the 12 
      embryonal and two pleomorphic RMS cases. The PAX7-FKHR fusion transcript was 
      detected in one case of embryonal RMS. CONCLUSIONS: The results indicate that the 
      RT-PCR assay is a reliable method for the detection of the PAX3-FKHR fusion 
      transcript of alveolar RMS in formalin-fixed, paraffin-embedded tissue. This 
      simple method enables pathologists to identify chromosomal rearrangements in RMS 
      as a diagnostic aid in cases where fresh or frozen tissue is not available.
FAU - Chen, B F
AU  - Chen BF
AD  - Department of Pathology, Mackay Memorial Hospital, Taipei, Taiwan, ROC.
FAU - Chen, M L
AU  - Chen ML
FAU - Liang, D C
AU  - Liang DC
FAU - Huang, Y W
AU  - Huang YW
FAU - Liu, H C
AU  - Liu HC
FAU - Chen, S H
AU  - Chen SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China (Republic : 1949- )
TA  - Zhonghua Yi Xue Za Zhi (Taipei)
JT  - Zhonghua yi xue za zhi = Chinese medical journal; Free China ed
JID - 0005327
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (FOXO1 protein, human)
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (PAX3 Transcription Factor)
RN  - 0 (PAX3 protein, human)
RN  - 0 (PAX7 Transcription Factor)
RN  - 0 (PAX7 protein, human)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 138016-91-8 (Pax3 protein, mouse)
RN  - 8002-74-2 (Paraffin)
SB  - IM
MH  - DNA-Binding Proteins/*genetics
MH  - Forkhead Box Protein O1
MH  - Forkhead Transcription Factors
MH  - *Homeodomain Proteins
MH  - Humans
MH  - Muscle Proteins/*genetics
MH  - Nerve Tissue Proteins/*genetics
MH  - PAX3 Transcription Factor
MH  - PAX7 Transcription Factor
MH  - Paired Box Transcription Factors
MH  - Paraffin
MH  - RNA, Messenger/*analysis
MH  - Recombinant Fusion Proteins/*genetics
MH  - *Reverse Transcriptase Polymerase Chain Reaction
MH  - Rhabdomyosarcoma/*genetics
MH  - Tissue Embedding
MH  - Transcription Factors/*genetics
MH  - Translocation, Genetic
EDAT- 1999/03/04 00:00
MHDA- 1999/03/04 00:01
CRDT- 1999/03/04 00:00
PHST- 1999/03/04 00:00 [pubmed]
PHST- 1999/03/04 00:01 [medline]
PHST- 1999/03/04 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi (Taipei). 1999 Feb;62(2):86-91.