PMID- 10064082
OWN - NLM
STAT- MEDLINE
DCOM- 19990316
LR  - 20180425
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 2
DP  - 1999 Feb
TI  - Induction of IFN-gamma-producing CD4+ natural killer T cells by Mycobacterium 
      bovis bacillus Calmette Guerin.
PG  - 650-9
AB  - The CD4+ natural killer (NK)T cells in the liver are potent IL-4 producers and 
      hence may promote Th2 cell development. Following Mycobacterium bovis bacillus 
      Calmette Guerin (BCG) infection, IL-4-producing CD4+ NKT cells become 
      undetectable in liver mononuclear cells of normal density (interface between 40 
      and 70% Percoll) by flow cytometry. The present study shows that M. bovis BCG 
      infection changes the density of liver CD4+ NKT cells and shifts cytokine 
      production from IL-4 to IFN-gamma. The number of CD4+ NK1+ TCR 
      alpha/beta(intermediate) cells increased in the low-density fraction (<40% 
      Percoll density gradient) in parallel to the reduction of this cell population in 
      the fraction of normal density. The number of IL-4-producing cells, however, was 
      small and high frequencies of IFN-gamma-secreting cells were identified in the 
      low-density fraction after TCR/CD3 ligation. Accordingly, selected low-density 
      CD4+ NKT cells encompassed high numbers of IFN-gamma producers and minute numbers 
      of IL-4-secreting cells. Induction of low-density CD4+ NKT cells by M. bovis BCG 
      was abrogated by endogenous IL-12 neutralization which also caused increased 
      bacterial growth in the liver. We assume that M. bovis BCG infection changes 
      cytokine secretion by the CD4+ NKT cell population from IL-4 to IFN-gamma through 
      IL-12 induction. Thus, CD4+ NKT cells may contribute to host resistance against 
      intracellular bacteria prior to conventional IFN-gamma-producing Th1 cells.
FAU - Emoto, M
AU  - Emoto M
AD  - Department of Immunology, Max-Planck-Institute for Infection Biology, Berlin, 
      Germany.
FAU - Emoto, Y
AU  - Emoto Y
FAU - Buchwalow, I B
AU  - Buchwalow IB
FAU - Kaufmann, S H
AU  - Kaufmann SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (CD4 Antigens)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - CD4 Antigens/immunology
MH  - *Immunity, Innate
MH  - Interferon-gamma/biosynthesis/*immunology
MH  - Interleukin-4/immunology
MH  - Killer Cells, Natural/*immunology
MH  - Liver/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mycobacterium bovis/*immunology
MH  - Receptors, Antigen, T-Cell, alpha-beta/immunology
MH  - Tuberculosis/*immunology/*veterinary
EDAT- 1999/03/04 03:38
MHDA- 2000/06/20 09:00
CRDT- 1999/03/04 03:38
PHST- 1999/03/04 03:38 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/03/04 03:38 [entrez]
AID - 10.1002/(SICI)1521-4141(199902)29:02<650::AID-IMMU650>3.0.CO;2-M [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Feb;29(2):650-9. doi: 
      10.1002/(SICI)1521-4141(199902)29:02<650::AID-IMMU650>3.0.CO;2-M.