PMID- 10065424
OWN - NLM
STAT- MEDLINE
DCOM- 19990422
LR  - 20071114
IS  - 1071-5576 (Print)
IS  - 1071-5576 (Linking)
VI  - 6
IP  - 1
DP  - 1999 Jan-Feb
TI  - Genome scanning detects amplification of the cathepsin B gene (CtsB) in 
      transformed rat ovarian surface epithelial cells.
PG  - 32-40
AB  - OBJECTIVE: To isolate a portion of the amplicon inferred to be present in a 
      malignant rat tumor cell line, NuTu 26, by the presence of a homogeneously 
      staining chromosomal region (hsr) and identify genes embedded within it. METHODS: 
      Genome scanning was used to identify an EcoRI fragment (8.6 kbp) within the 
      amplified region of the NuTu 26 genome using a recently identified rat repetitive 
      sequence, OST17 as a probe. The 8.6 kbp amplified fragment was sequenced and used 
      as starting material to obtain additional sequence information by screening a P1 
      clone-derived DNA library to identify any genes likely embedded in the amplicon. 
      Use of the microdissected hsr as a probe for fluorescence in situ hybridization 
      (FISH) and application of Southern, Northern, and Western blot analysis confirmed 
      the amplification of this region in the NuTu 26 genome. RESULTS: The cathepsin B 
      gene was within the amplicon of the hsr-containing marker chromosome of NuTu 26. 
      FISH analysis and chromosomal banding further revealed that the marker chromosome 
      was a derivative of chromosomes 4 and 15, i.e., der(15)t(4;15). CONCLUSION: 
      Cathepsin B gene amplification may contribute to some aspect of the biology of 
      ovarian cancer. This concept is strengthened by the finding that the gene is 
      overexpressed frequently in independently transformed rat ovarian surface 
      epithelial cells.
FAU - Abdollahi, A
AU  - Abdollahi A
AD  - Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111, 
      USA.
FAU - Getts, L A
AU  - Getts LA
FAU - Sonoda, G
AU  - Sonoda G
FAU - Miller, P D
AU  - Miller PD
FAU - Taguchi, T
AU  - Taguchi T
FAU - Godwin, A K
AU  - Godwin AK
FAU - Testa, J R
AU  - Testa JR
FAU - Hamilton, T C
AU  - Hamilton TC
LA  - eng
GR  - CA56916/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Soc Gynecol Investig
JT  - Journal of the Society for Gynecologic Investigation
JID - 9433806
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.21.- (Deoxyribonuclease EcoRI)
RN  - EC 3.4.22.1 (Cathepsin B)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cathepsin B/*genetics
MH  - Cell Line, Transformed
MH  - DNA/analysis/metabolism
MH  - Deoxyribonuclease EcoRI/metabolism
MH  - Epithelium
MH  - Female
MH  - *Gene Amplification
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Ovarian Neoplasms/*genetics
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Repetitive Sequences, Nucleic Acid
MH  - Restriction Mapping
MH  - Tumor Cells, Cultured
EDAT- 1999/03/05 00:00
MHDA- 1999/03/05 00:01
CRDT- 1999/03/05 00:00
PHST- 1999/03/05 00:00 [pubmed]
PHST- 1999/03/05 00:01 [medline]
PHST- 1999/03/05 00:00 [entrez]
AID - S1071-5576(98)00048-3 [pii]
PST - ppublish
SO  - J Soc Gynecol Investig. 1999 Jan-Feb;6(1):32-40.