PMID- 10065460
OWN - NLM
STAT- MEDLINE
DCOM- 19990421
LR  - 20131121
IS  - 0006-8969 (Print)
IS  - 0006-8969 (Linking)
VI  - 51
IP  - 1
DP  - 1999 Jan
TI  - [Ibudilast prevents oxygen-glucose deprivation-induced oligodendroglial injury].
PG  - 49-54
AB  - Previously we have demonstrated that ibudilast, which is used clinically for 
      treating patients with asthma and cerebrovascular diseases, prevents 
      excitotoxicity of oligodendroglial lineage mediated by Ca2+ influx via 
      non-N-methyl-D-aspartate (NMDA) glutamate receptor (GluR) channels. We here 
      present a finding that ibudilast prevents oxygen-glucose deprivation 
      (OGD)-induced oligodendroglial injury. The oligodendrocyte-like cells (OLC), 
      differentiated from the CG-4 cell line established from rat 
      oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells, were exposed to hypoxia 
      in the absence of glucose for 12 h and subsequent reoxygenation for 2 h. Cell 
      damage was evaluated by measuring activity of lactate dehydrogenase (LDH) 
      released into the culture medium. OGD for 12 h induced 30 to 50% LDH release into 
      the medium. OLC damage induced by deprivation of oxygen and glucose was prevented 
      by ibudilast at concentrations of > or = 50 microM. The protection given by 
      ibudilast against OGD-induced injury was enhanced by prostacyclin (PGI2). 
      OGD-induced OLC injury was prevented by 6-cyano-7-nitroquinoxaline-2, 3-dione 
      (CNQX), an inhibitor of non-NMDA GluR or deprivation of Ca2+ from culture medium. 
      While ibudilast increased intracellular cAMP at concentrations of > or = 10 
      microM, at least 100 microM concentrations were needed to increase intracellular 
      cGMP. Therefore, we concluded that ibudilast prevented OGD-induced 
      oligodendroglial injury possibly by increasing intracellular cAMP which modulates 
      Ca2+ influx via non-NMDA GluR channels.
FAU - Yoshioka, A
AU  - Yoshioka A
AD  - Department of Neurology, Kanazawa Medical University, Ishikawa, Japan.
FAU - Yamaya, Y
AU  - Yamaya Y
FAU - Hirose, G
AU  - Hirose G
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - No To Shinkei
JT  - No to shinkei = Brain and nerve
JID - 0413550
RN  - 0 (Bronchodilator Agents)
RN  - 0 (Pyridines)
RN  - 0 (Vasodilator Agents)
RN  - DCR9Z582X0 (Epoprostenol)
RN  - E0399OZS9N (Cyclic AMP)
RN  - H2D2X058MU (Cyclic GMP)
RN  - IY9XDZ35W2 (Glucose)
RN  - M0TTH61XC5 (ibudilast)
SB  - IM
MH  - Animals
MH  - Bronchodilator Agents/*pharmacology
MH  - Cell Hypoxia/*drug effects
MH  - Cells, Cultured
MH  - Cyclic AMP/analysis
MH  - Cyclic GMP/analysis
MH  - Epoprostenol/pharmacology
MH  - Glucose/*metabolism
MH  - Oligodendroglia/*drug effects
MH  - Pyridines/pharmacology
MH  - Rats
MH  - Vasodilator Agents/*pharmacology
EDAT- 1999/03/05 00:00
MHDA- 1999/03/05 00:01
CRDT- 1999/03/05 00:00
PHST- 1999/03/05 00:00 [pubmed]
PHST- 1999/03/05 00:01 [medline]
PHST- 1999/03/05 00:00 [entrez]
PST - ppublish
SO  - No To Shinkei. 1999 Jan;51(1):49-54.