PMID- 10066764
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 11
DP  - 1999 Mar 12
TI  - Nuclear factor I-mediated repression of the mouse mammary tumor virus promoter is 
      abrogated by the coactivators p300/CBP and SRC-1.
PG  - 7072-81
AB  - To better understand the function of nuclear factor I (NFI) proteins in 
      transcription, we have used transient transfection assays to assess 
      transcriptional modulation by NFI proteins on the NFI-dependent mouse mammary 
      tumor virus (MMTV) promoter. Expression of NFI-C or NFI-X, but not NFI-A or NFI-B 
      proteins, represses glucocorticoid induction of the MMTV promoter in HeLa cells. 
      Repression is DNA binding-independent as a deletion construct expressing the 
      NH2-terminal 160 residues of NFI-C represses but does not bind DNA. Repression by 
      NFI-C is cell type-dependent and occurs in HeLa and COS-1 cells but not 293 or 
      JEG-3 cells. NFI-C does not repress progesterone induction of the MMTV promoter 
      in HeLa cells, suggesting that progesterone induction of the promoter differs 
      mechanistically from glucocorticoid induction. NFI-C-mediated repression is 
      alleviated by overexpression of glucocorticoid receptor (GR), suggesting that 
      NFI-C represses the MMTV promoter by preventing GR function. However, repression 
      by NFI-C occurs with only a subset of glucocorticoid-responsive promoters, as the 
      chimeric NFIGREbeta-gal promoter that is activated by GR is not repressed by 
      NFI-C. Since the coactivator proteins p300/CBP, SRC-1A, and RAC3 had previously 
      been shown to function at steroid hormone-responsive promoters, we asked whether 
      they could influence NFI-C-mediated repression of MMTV expression. Expression of 
      p300/CBP or SRC-1A alleviates repression by NFI-C, whereas RAC3 has no effect. 
      This abrogation of NFI-C-mediated repression by p300/CBP and SRC-1A suggests that 
      repression by NFI-C may occur by interference with coactivator function at the 
      MMTV promoter.
FAU - Chaudhry, A Z
AU  - Chaudhry AZ
AD  - Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic 
      Foundation, Cleveland, Ohio 44195, USA.
FAU - Vitullo, A D
AU  - Vitullo AD
FAU - Gronostajski, R M
AU  - Gronostajski RM
LA  - eng
GR  - HD34908/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Glucocorticoids)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (NFIA protein, human)
RN  - 0 (NFIC protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Y-Box-Binding Protein 1)
RN  - 0 (YBX1 protein, human)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Ncoa1 protein, mouse)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - Animals
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - Cell Line
MH  - DNA-Binding Proteins/*metabolism
MH  - Glucocorticoids/pharmacology
MH  - Histone Acetyltransferases
MH  - Humans
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - NFI Transcription Factors
MH  - Nuclear Proteins/*metabolism
MH  - Nuclear Receptor Coactivator 1
MH  - Progesterone/pharmacology
MH  - *Promoter Regions, Genetic
MH  - Receptors, Glucocorticoid/metabolism
MH  - Repetitive Sequences, Nucleic Acid
MH  - Repressor Proteins/*metabolism
MH  - Trans-Activators/*metabolism
MH  - Transcription Factors/*metabolism
MH  - Y-Box-Binding Protein 1
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
AID - S0021-9258(18)36975-8 [pii]
AID - 10.1074/jbc.274.11.7072 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Mar 12;274(11):7072-81. doi: 10.1074/jbc.274.11.7072.