PMID- 10067698
OWN - NLM
STAT- MEDLINE
DCOM- 19990527
LR  - 20190728
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 17
IP  - 7-8
DP  - 1999 Feb 26
TI  - A historical and molecular phylogeny of BCG strains.
PG  - 915-22
AB  - Bacillus Calmette-Guerin (BCG) is the name given to a family of vaccines derived 
      in 1921 by the in-vitro attenuation of Mycobacterium bovis. Subsequently, BCG 
      seed lots were distributed globally, and both phenotypic and genotypic 
      differences between strains have been described. As a step to understanding BCG 
      diversity, we have reviewed the English and French historical record on 13 
      strains and DNA was extracted from these strains for 
      restriction-fragment-length-polymorphism study. The written record suggests 
      "early strains" obtained from the Pasteur Institute between 1924 and 1926 and 
      "later strains" obtained after 1931. Molecular typing resulted in 3 clades, based 
      on variability in IS6110-typing and the presence of mpt64 gene. With two 
      exceptions, these clades correspond with strains obtained in 1924-26 
      (IS6110-2/mpt64+), 1926-31 (IS6110-1/mpt64+), and 1931 or later (IS6110-1/mpt64-) 
      This analysis demonstrates that BCG has undergone genetic changes since 1921, 
      consistent with ongoing in-vitro evolution.
FAU - Behr, M A
AU  - Behr MA
AD  - Department of Medicine, Stanford University School of Medicine, CA, USA. 
      marcel@molepi.stanford.edu
FAU - Small, P M
AU  - Small PM
LA  - eng
GR  - A101137/PHS HHS/United States
GR  - A134238/PHS HHS/United States
PT  - Comparative Study
PT  - Historical Article
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (BCG Vaccine)
SB  - IM
MH  - BCG Vaccine/*history
MH  - Europe
MH  - History, 20th Century
MH  - Mycobacterium bovis/*classification/*genetics
MH  - *Phylogeny
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
AID - S0264-410X(98)00277-1 [pii]
AID - 10.1016/s0264-410x(98)00277-1 [doi]
PST - ppublish
SO  - Vaccine. 1999 Feb 26;17(7-8):915-22. doi: 10.1016/s0264-410x(98)00277-1.