PMID- 10067827
OWN - NLM
STAT- MEDLINE
DCOM- 19990323
LR  - 20091119
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 140
IP  - 3
DP  - 1999 Mar
TI  - Fibroblast growth factor-2 induces hepatocyte growth factor/scatter factor 
      expression in osteoblasts.
PG  - 1069-74
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional growth 
      factor with a major role in tissue morphogenesis and repair. It stimulates the 
      proliferation of cells of the osteoblast and osteoclast lineages. Mitogenic 
      factors playing a role in fracture repair may act by regulating HGF/SF expression 
      or activity in bone-forming cells. We investigated the effect of fibroblast 
      growth factor-2 (FGF-2) on the expression of HGF/SF and its receptor, encoded by 
      c-met, in the MC3T3-E1 osteoblastic cell line. MC3T3-E1 cells expressed low 
      levels of HGF/SF messenger RNA (mRNA), which were markedly increased by FGF-2 in 
      a dose- and time-dependent manner. FGF-2 also induced HGF/SF polypeptide 
      synthesis. The stimulation of HGF/SF mRNA expression by FGF-2 was blocked by 
      cycloheximide, a protein synthesis inhibitor, but not by DNA or prostaglandin 
      synthesis inhibitors. FGF-2 increased the rate of HGF/SF gene transcription by 
      approximately 2-fold, as determined by nuclear run-on assays, and did not modify 
      the decay of HGF/SF mRNA in transcriptionally arrested cells. FGF-2 also caused a 
      dose- and time-dependent stimulation of c-met mRNA. In conclusion, FGF-2 induces 
      HGF/SF expression in osteoblasts and may promote HGF/SF activity by increasing 
      the expression of its receptor. Through these mechanisms, HGF/SF could mediate 
      FGF actions on bone repair.
FAU - Blanquaert, F
AU  - Blanquaert F
AD  - Department of Research, Saint Francis Hospital and Medical Center, Hartford, 
      Connecticut 06105, USA.
FAU - Delany, A M
AU  - Delany AM
FAU - Canalis, E
AU  - Canalis E
LA  - eng
GR  - AR-21707/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Line
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Hepatocyte Growth Factor/*biosynthesis
MH  - Mice
MH  - Osteoblasts/*drug effects/metabolism
MH  - Proto-Oncogene Proteins c-met/*biosynthesis
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
AID - 10.1210/endo.140.3.6553 [doi]
PST - ppublish
SO  - Endocrinology. 1999 Mar;140(3):1069-74. doi: 10.1210/endo.140.3.6553.