PMID- 10067860
OWN - NLM
STAT- MEDLINE
DCOM- 19990323
LR  - 20081121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 140
IP  - 3
DP  - 1999 Mar
TI  - Cloning, tissue expression, and chromosomal location of the mouse insulin 
      receptor substrate 4 gene.
PG  - 1329-37
AB  - The insulin receptor substrates (IRSs) are key proteins in signal transduction 
      from the insulin receptor. Recently, we discovered a fourth member of this 
      family, designated IRS-4, cloned its complementary DNA from the human embryonic 
      kidney 293 cell line, and characterized its signaling properties in this cell 
      line. As part of an investigation of the physiological role of this IRS, we have 
      now cloned the mouse IRS-4 gene and determined its tissue expression and 
      chromosomal location. The coding region of the mouse IRS-4 gene contains no 
      introns, and in this regard is the same as that of the genes for IRS-1 and -2. 
      The predicted amino acid sequence of mouse IRS-4 is highly homologous with that 
      of human IRS-4; the pleckstrin homology domain, the phosphotyrosine-binding 
      domain, and the tyrosine phosphorylation motifs are especially well conserved. 
      The tissue distribution of IRS-4 in the mouse was determined by analysis for the 
      expression of its messenger RNA by RT-PCR and for the protein itself by 
      immunoprecipitation and immunoblotting. The messenger RNA was detected in 
      skeletal muscle, brain, heart, kidney, and liver, but the protein itself was not 
      detected in any tissue. These results indicate that IRS-4 is a very rare protein. 
      The chromosomal locations of the mouse IRS-4 and IRS-3 genes were determined by 
      interspecific back-cross analysis and were found to be on chromosomes X and 5, 
      respectively. As the mouse genes for IRS-1 and -2 are on chromosomes 1 and 8, 
      respectively, each IRS gene resides on a different chromosome.
FAU - Fantin, V R
AU  - Fantin VR
AD  - Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 
      03755, USA.
FAU - Lavan, B E
AU  - Lavan BE
FAU - Wang, Q
AU  - Wang Q
FAU - Jenkins, N A
AU  - Jenkins NA
FAU - Gilbert, D J
AU  - Gilbert DJ
FAU - Copeland, N G
AU  - Copeland NG
FAU - Keller, S R
AU  - Keller SR
FAU - Lienhard, G E
AU  - Lienhard GE
LA  - eng
GR  - DK-42816/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (IRS4 protein, human)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Irs4 protein, mouse)
RN  - 0 (Phosphoproteins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - Embryonic and Fetal Development/physiology
MH  - Gene Expression Regulation/*physiology
MH  - Humans
MH  - Insulin Receptor Substrate Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phosphoproteins/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
AID - 10.1210/endo.140.3.6578 [doi]
PST - ppublish
SO  - Endocrinology. 1999 Mar;140(3):1329-37. doi: 10.1210/endo.140.3.6578.