PMID- 10067960
OWN - NLM
STAT- MEDLINE
DCOM- 19990310
LR  - 20171116
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 40
IP  - 3
DP  - 1999 Mar
TI  - Effect of leukocytes on corneal cellular proliferation and wound healing.
PG  - 575-81
AB  - PURPOSE: To establish whether fucoidin, by blocking the adhesion of leukocytes on 
      the limbal vascular endothelium, prevents extravasation of the cells from the 
      blood stream into the limbal stroma and the wounded area after corneal injury. 
      Successful leukocyte blocking enabled investigation of the influence of 
      leukocytes on corneal cellular proliferation after corneal wounding. METHODS: 
      Thirty-two New Zealand White rabbits were used. Photorefractive keratectomy (PRK) 
      and a standardized alkali corneal wound were used as models in two sets of 
      experiments. In half of the injured rabbits fucoidin was used to prevent 
      leukocytes from leaving the local vessels. The efficiency of the blocking 
      technique was evaluated by counting the number of leukocytes in the limbal and 
      wounded corneal areas. Proliferating cell nuclear antigen (PCNA) was used as a 
      marker for proliferative activity. RESULTS: The infiltration of leukocytes into 
      the limbus and the cornea after PRK and alkali injuries can be blocked by 
      fucoidin. The healing rate of corneal epithelium after alkali burn was retarded 
      in the absence of leukocytes. PCNA expression was enhanced in the presence of 
      leukocytes. Fucoidin per se had no influence on corneal cell proliferation and 
      wound healing. CONCLUSIONS: Polymorphonuclear leukocytes (PMNs) can be prevented 
      from entering the cornea in vivo by fucoidin after PRK and after alkali burn. The 
      corneal epithelial healing rate is delayed in the absence of PMNs in vivo, and 
      PCNA expression increases in the presence of leukocytes.
FAU - Gan, L
AU  - Gan L
AD  - St. Erik's Eye Hospital, Karolinska Institute, Stockholm, Sweden.
FAU - Fagerholm, P
AU  - Fagerholm P
FAU - Kim, H J
AU  - Kim HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Polysaccharides)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 28RYY2IV3F (Fucose)
RN  - 9072-19-9 (fucoidan)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Cell Adhesion/drug effects
MH  - Cell Adhesion Molecules/drug effects
MH  - Cell Division/drug effects
MH  - Chemotaxis, Leukocyte/*drug effects/physiology
MH  - Cornea/*drug effects/metabolism
MH  - Corneal Injuries
MH  - Epithelial Cells/physiology
MH  - Eye Injuries/*drug therapy/metabolism
MH  - Fucose/administration & dosage/*pharmacology
MH  - Injections, Intravenous
MH  - Neutrophils/*drug effects/physiology
MH  - Polysaccharides/administration & dosage/*pharmacology
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - Rabbits
MH  - Wound Healing/*drug effects/physiology
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1999 Mar;40(3):575-81.