PMID- 10068024
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190915
IS  - 0301-620X (Print)
IS  - 0301-620X (Linking)
VI  - 81
IP  - 1
DP  - 1999 Jan
TI  - Induction of macrophage C-C chemokine expression by titanium alloy and bone 
      cement particles.
PG  - 155-62
AB  - Particulate wear debris is associated with periprosthetic inflammation and 
      loosening in total joint arthroplasty. We tested the effects of titanium alloy 
      (Ti-alloy) and PMMA particles on monocyte/macrophage expression of the C-C 
      chemokines, monocyte chemoattractant protein-1 (MCP-1), monocyte inflammatory 
      protein-1 alpha (MIP-1alpha), and regulated upon activation normal T expressed 
      and secreted protein (RANTES). Periprosthetic granulomatous tissue was analysed 
      for expression of macrophage chemokines by immunohistochemistry. Chemokine 
      expression in human monocytes/macrophages exposed to Ti-alloy and PMMA particles 
      in vitro was determined by RT-PCR, ELISA and monocyte migration. We observed 
      MCP-1 and MIP-1alpha expression in all tissue samples from failed arthroplasties. 
      Ti-alloy and PMMA particles increased expression of MCP-1 and MIP-1alpha in 
      macrophages in vitro in a dose- and time-dependent manner whereas RANTES was not 
      detected. mRNA signal levels for MCP-1 and MIP-1alpha were also observed in cells 
      after exposure to particles. Monocyte migration was stimulated by culture medium 
      collected from macrophages exposed to Ti-alloy and PMMA particles. Antibodies to 
      MCP-1 and MIP-1alpha inhibited chemotactic activity of the culture medium 
      samples. Release of C-C chemokines by macrophages in response to wear particles 
      may contribute to chronic inflammation at the bone-implant interface in total 
      joint arthroplasty.
FAU - Nakashima, Y
AU  - Nakashima Y
AD  - Stanford University School of Medicine, California, USA.
FAU - Sun, D H
AU  - Sun DH
FAU - Trindade, M C
AU  - Trindade MC
FAU - Chun, L E
AU  - Chun LE
FAU - Song, Y
AU  - Song Y
FAU - Goodman, S B
AU  - Goodman SB
FAU - Schurman, D J
AU  - Schurman DJ
FAU - Maloney, W J
AU  - Maloney WJ
FAU - Smith, R L
AU  - Smith RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Bone Joint Surg Br
JT  - The Journal of bone and joint surgery. British volume
JID - 0375355
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Bone Cements)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines, CC)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Bone Cements
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CCL5
MH  - Chemokines, CC/*metabolism
MH  - Chemotaxis
MH  - *Hip Prosthesis
MH  - Humans
MH  - Macrophage Inflammatory Proteins/metabolism
MH  - Macrophages/*metabolism
MH  - Osteolysis/*metabolism/pathology
MH  - Prosthesis Failure
MH  - Titanium
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
AID - 10.1302/0301-620x.81b1.8884 [doi]
PST - ppublish
SO  - J Bone Joint Surg Br. 1999 Jan;81(1):155-62. doi: 10.1302/0301-620x.81b1.8884.