PMID- 10068207
OWN - NLM
STAT- MEDLINE
DCOM- 19990401
LR  - 20220227
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 79
IP  - 2
DP  - 1999 Feb
TI  - Prevention of myocardial reperfusion injury in rats by an antibody against 
      monocyte chemotactic and activating factor/monocyte chemoattractant protein-1.
PG  - 195-203
AB  - MCAF (monocyte chemotactic and activating factor)/MCP-1 (monocyte chemoattractant 
      protein-1) is an important mediator of monocyte recruitment to inflammatory 
      sites. However, its pathophysiologic role in myocardial reperfusion injury 
      remains unknown. Male Wistar rats were anesthetized, and the left anterior 
      descending coronary artery was ligated for an hour, after which the ligature was 
      released. Northern blotting analysis revealed that MCAF/MCP-1 mRNA expression 
      increased 16-fold in the reperfused region at 12 hours after reperfusion. 
      MCAF/MCP-1 concentration in plasma and the heart was already elevated after hour 
      of ischemia in this model. Goat polyclonal antibodies were prepared by repeated 
      immunization of animals with purified, recombinant rat MCAF/MCP-1, and the 
      neutralizing activities of this antibody were confirmed by monocyte chemotaxis 
      assay and administration to rats with crescentic glomerulonephritis. Intravenous 
      injection of anti-MCAF/MCP-1 antibody significantly reduced the infarct size at 
      24 hours after reperfusion compared with the injection of control IgG (33.9 +/- 
      5.1% vs 49.4 +/- 2.7% of ischemic area, mean +/- SEM). Administration of this 
      antibody markedly decreased the intercellular adhesion molecule-1 mRNA expression 
      and infiltration of macrophages, which suggested the pathophysiologic role of 
      MCAF/MCP-1. Neutralization of MCAF/MCP-1 is beneficial by preventing reperfusion 
      injury in a rat model of myocardial ischemia and reperfusion.
FAU - Ono, K
AU  - Ono K
AD  - Department of Cardiovascular Medicine, Kyoto University, Japan.
FAU - Matsumori, A
AU  - Matsumori A
FAU - Furukawa, Y
AU  - Furukawa Y
FAU - Igata, H
AU  - Igata H
FAU - Shioi, T
AU  - Shioi T
FAU - Matsushima, K
AU  - Matsushima K
FAU - Sasayama, S
AU  - Sasayama S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Antibodies)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Antibodies/*therapeutic use
MH  - Cell Movement/drug effects/physiology
MH  - Chemokine CCL2/blood/genetics/*immunology
MH  - Intercellular Adhesion Molecule-1/genetics
MH  - Macrophages/drug effects/physiology
MH  - Male
MH  - Myocardial Infarction/pathology
MH  - Myocardial Reperfusion Injury/*prevention & control
MH  - Osmolar Concentration
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 1999/03/06 00:00
MHDA- 1999/03/06 00:01
CRDT- 1999/03/06 00:00
PHST- 1999/03/06 00:00 [pubmed]
PHST- 1999/03/06 00:01 [medline]
PHST- 1999/03/06 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1999 Feb;79(2):195-203.