PMID- 10068521
OWN - NLM
STAT- MEDLINE
DCOM- 19990325
LR  - 20061115
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 82
IP  - 1
DP  - 1999 Mar
TI  - Reduced capacity to inhibit elastase in abdominal aortic aneurysm.
PG  - 24-7
AB  - BACKGROUND: Loss of elastin in the aortic wall is an early event in abdominal 
      aortic aneurysm (AAA). An imbalance in the protease-antiprotease system is 
      proposed to be one of the factors that promote connective tissue destruction. We 
      hypothesize that plasma from AAA patients will have a reduced inhibitory capacity 
      in comparison to normal controls. MATERIALS AND METHODS: Using an assay we 
      developed, plasma (10 microliters), collected from AAA patients (n = 14) and 
      normal controls (n = 13), was added to the elastase inhibition assay containing 
      succinylated elastin substrate. The reaction was initiated with 13.9 units 
      porcine pancreatic elastase (PPE). Elastase activity in the presence and absence 
      of plasma was compared. Plasma elastase was also determined using the Merck 
      PMN-elastase kit. RESULTS: The relative activity of exogenous elastase (%) in the 
      presence of AAA plasma (n = 14, mean age 73.4 years +/- 1.7 SEM) was 42.59% +/- 
      4.3 SEM, while that in the presence of control plasma (n = 13, mean age 73.9 
      years +/- 2.1 SEM) was 10.23% +/- 2.1 SEM (P < 0.0001). Analysis of plasma 
      elastase (microgram/L) indicated that there was no significant difference between 
      normal (n = 9, 207.33 microgram/L +/- 58.67 SEM) and AAA (n = 9, 145.34 
      microgram/L +/- 29.54 SEM) (P = 0.359). CONCLUSION: There is a significant 
      reduction in the plasma inhibitory capacity of elastase in AAA patients in 
      comparison to normal controls, though plasma elastase level was not significantly 
      different. The data presented here give experimental evidence to the 
      protease-antiprotease imbalance in AAA patient plasma and may lead to the 
      development of a measurable parameter to monitor AAA.
CI  - Copyright 1999 Academic Press.
FAU - Rao, S K
AU  - Rao SK
AD  - Department of Surgery, Long Island Jewish Medical Center, Long Island Campus for 
      the Albert Einstein College of Medicine, New Hyde Park, New York, 11042, USA. 
      rao@lij.edu
FAU - Mathrubutham, M
AU  - Mathrubutham M
FAU - Sherman, D
AU  - Sherman D
FAU - Cerveira, J
AU  - Cerveira J
FAU - Cohen, J R
AU  - Cohen JR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Enzyme Inhibitors)
RN  - 9007-58-3 (Elastin)
RN  - EC 3.4.21.36 (Pancreatic Elastase)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
MH  - Aged
MH  - Aortic Aneurysm, Abdominal/*enzymology/etiology/metabolism
MH  - Case-Control Studies
MH  - Connective Tissue/metabolism
MH  - Elastic Tissue/metabolism
MH  - Elastin/deficiency
MH  - Enzyme Inhibitors/blood
MH  - Humans
MH  - Metalloendopeptidases/metabolism
MH  - Pancreatic Elastase/*antagonists & inhibitors
EDAT- 1999/03/09 00:00
MHDA- 1999/03/09 00:01
CRDT- 1999/03/09 00:00
PHST- 1999/03/09 00:00 [pubmed]
PHST- 1999/03/09 00:01 [medline]
PHST- 1999/03/09 00:00 [entrez]
AID - S0022-4804(98)95515-9 [pii]
AID - 10.1006/jsre.1998.5515 [doi]
PST - ppublish
SO  - J Surg Res. 1999 Mar;82(1):24-7. doi: 10.1006/jsre.1998.5515.