PMID- 10069419
OWN - NLM
STAT- MEDLINE
DCOM- 19990817
LR  - 20190513
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 11
IP  - 2
DP  - 1999 Feb
TI  - Characterization of the culture filtrate-specific cytotoxic T lymphocyte response 
      induced by Bacillus Calmette-Guerin vaccination in H-2b mice.
PG  - 209-16
AB  - Although CD8+ T cells are supposed to play an important role in protective 
      immunity to mycobacteria, cytotoxic T lymphocyte (CTL) responses in this 
      infection remain poorly characterized. We previously demonstrated that bacillus 
      Calmette-Guerin (BCG) immunization of H-2b mice induced CTL able to recognize and 
      kill macrophages incubated with proteins from mycobacterial culture supernatant 
      [culture filtrate (CF) antigens]. In the present study, we have further 
      characterized the lytic activity of these CTL and the processing pathway used for 
      the presentation of CF proteins. We show that they use the degranulation pathway 
      (secretion of perforins and granzymes) as the main lytic mechanism of 
      cytotoxicity and also secrete IFN-gamma upon incubation with CF-pulsed 
      macrophages. The in vitro presentation of CF proteins to CTL required a 
      processing step inhibited in the cold but insensitive to Brefeldin A. 
      Transporter-associated protein (TAP)-2-deficient RMA-S cells were efficiently 
      recognized and killed by CF-specific CTL, demonstrating the lack of TAP 
      requirement for this presentation. However, recognition of target cells by CTL 
      was abolished when carried out in the presence of chloroquine. These results 
      indicate that a non-classical MHC class I-processing pathway allows the 
      recognition of a CF protein by CTL in BCG-vaccinated H-2b mice.
FAU - Denis, O
AU  - Denis O
AD  - Laboratory of Mycobacterial Immunology, Pasteur Institute of Brussels, Belgium.
FAU - Huygen, K
AU  - Huygen K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (BCG Vaccine)
RN  - 0 (Culture Media)
RN  - 0 (H-2 Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Pore Forming Cytotoxic Proteins)
RN  - 126465-35-8 (Perforin)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 886U3H6UFF (Chloroquine)
SB  - IM
MH  - Animals
MH  - BCG Vaccine/administration & dosage/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Line
MH  - Chloroquine/pharmacology
MH  - Culture Media
MH  - H-2 Antigens/*immunology
MH  - Interferon-gamma/biosynthesis
MH  - Lymphocyte Activation
MH  - Membrane Glycoproteins/biosynthesis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Perforin
MH  - Pore Forming Cytotoxic Proteins
MH  - T-Lymphocytes, Cytotoxic/drug effects/*immunology
EDAT- 1999/03/09 00:00
MHDA- 1999/03/09 00:01
CRDT- 1999/03/09 00:00
PHST- 1999/03/09 00:00 [pubmed]
PHST- 1999/03/09 00:01 [medline]
PHST- 1999/03/09 00:00 [entrez]
AID - 10.1093/intimm/11.2.209 [doi]
PST - ppublish
SO  - Int Immunol. 1999 Feb;11(2):209-16. doi: 10.1093/intimm/11.2.209.