PMID- 10069486
OWN - NLM
STAT- MEDLINE
DCOM- 19990318
LR  - 20190831
IS  - 0278-6915 (Print)
IS  - 0278-6915 (Linking)
VI  - 37
IP  - 1
DP  - 1999 Jan
TI  - Development of a non-radioactive endpoint in a modified local lymph node assay.
PG  - 87-93
AB  - A murine local lymph node assay (LLNA) has been developed as an alternative to 
      guinea pig models for contact sensitization testing. Although the LLNA appears to 
      be a little less sensitive than the most stringent of guinea pig assays, it 
      provides a rapid, objective, quantitative and cost-effective method for screening 
      strong contact sensitizers and has advantages with respect to animal welfare. 
      However, a potential disadvantage is the need for the use of radioactive 
      material. We have reported previously that an ex vivo assay based on similar 
      principles to the original in vivo LLNA, but using a non-radioactive endopoint, 
      was valid for the prediction of strong sensitizers. This ex vivo assay was not 
      sensitive enough to allow prediction of moderately potent ones. In this study, we 
      propose a new parameter, Corrected IL-2 Index (CII), for the prediction of 
      moderate sensitizers. To obtain CII the IL-2 release in the supernatant of the 
      cell culture is corrected for lymph node weight ratio and ratio of CD4-positive 
      subset. We found that CII predicted the allergenicity of moderate sensitizers, 
      including the ones recommended by the OECD in guideline 406, such as 
      mercaptobenzothiazole and hexyl cinnamic aldehyde. The allergenicity of metal 
      salts, such as potassium dichromate, ammonium tetrachloroplatinate and cobalt 
      chloride, was also predicted by the CII. We conclude that the use of CII as an 
      index significantly increases the sensitivity of the ex vivo method so that 
      moderate sensitizers may also be detected.
FAU - Hariya, T
AU  - Hariya T
AD  - Shiseido Skin Biology Research Laboratories, Yokohama, Japan.
FAU - Hatao, M
AU  - Hatao M
FAU - Ichikawa, H
AU  - Ichikawa H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Allergens)
RN  - 0 (Interleukin-2)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animals
MH  - Dermatitis, Contact/*etiology
MH  - Disease Models, Animal
MH  - Female
MH  - Interleukin-2/metabolism
MH  - Lymph Nodes/diagnostic imaging/*drug effects/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Radionuclide Imaging
MH  - Sensitivity and Specificity
EDAT- 1999/03/09 00:00
MHDA- 1999/03/09 00:01
CRDT- 1999/03/09 00:00
PHST- 1999/03/09 00:00 [pubmed]
PHST- 1999/03/09 00:01 [medline]
PHST- 1999/03/09 00:00 [entrez]
AID - S0278-6915(98)00102-1 [pii]
AID - 10.1016/s0278-6915(98)00102-1 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 1999 Jan;37(1):87-93. doi: 10.1016/s0278-6915(98)00102-1.