PMID- 10070115
OWN - NLM
STAT- MEDLINE
DCOM- 19990415
LR  - 20190205
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 276
IP  - 3
DP  - 1999 Mar
TI  - Expression of heat shock protein 72 by alveolar macrophages in hypersensitivity 
      pneumonitis.
PG  - L501-5
LID - 10.1152/ajplung.1999.276.3.L501 [doi]
AB  - The current study was done to look at a possible role of heat shock proteins 
      (HSPs) in hypersensitivity pneumonitis (HP). The specific aims were to determine 
      whether there was a difference in the expression of HSP72 in alveolar macrophages 
      (AMs) between mice challenged with HP antigen and saline-treated control mice and 
      between AMs obtained by bronchoalveolar lavage from 18 patients with HP and 11 
      normal subjects. The expression of HSP72 was studied under basal conditions and 
      under a mild heat shock. HSP72 expression by AMs in response to in vitro 
      stimulation with Saccharopolyspora rectivirgula was lower in AMs of control mice 
      than in those of HP animals. HSP72 was constitutively expressed in AMs of both 
      normal and HP subjects. Densitometric ratios showed that AMs from normal subjects 
      responded to heat shock with a 39 degrees C-to-37 degrees C ratio of 1.72 +/- 
      0.18 (mean +/- SE), and AMs from HP patients responded with a ratio of 1.16 +/- 
      0.16 (P = 0.0377). This decreased induction by additional stress of AMs could 
      lead to an altered immunoregulatory activity and account for the inflammation 
      seen in HP.
FAU - Racine, C
AU  - Racine C
AD  - Unite de Recherche, Centre de Pneumologie, Institut de Cardiologie et de 
      Pneumologie, Hopital and Universite Laval, Sainte-Foy, Quebec, Canada G1V 4G5.
FAU - Israel-Assayag, E
AU  - Israel-Assayag E
FAU - Cormier, Y
AU  - Cormier Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Antigens, Bacterial)
RN  - 0 (HSP72 Heat-Shock Proteins)
RN  - 0 (Heat-Shock Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/immunology
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Cell Count
MH  - Female
MH  - HSP72 Heat-Shock Proteins
MH  - Heat-Shock Proteins/*metabolism
MH  - Humans
MH  - Macrophages, Alveolar/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pneumonia/*etiology/*metabolism/pathology
MH  - Respiratory Hypersensitivity/*complications/immunology
MH  - Saccharopolyspora/immunology
EDAT- 1999/03/10 00:00
MHDA- 1999/03/10 00:01
CRDT- 1999/03/10 00:00
PHST- 1999/03/10 00:00 [pubmed]
PHST- 1999/03/10 00:01 [medline]
PHST- 1999/03/10 00:00 [entrez]
AID - 10.1152/ajplung.1999.276.3.L501 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Mar;276(3):L501-5. doi: 10.1152/ajplung.1999.276.3.L501.