PMID- 10072396
OWN - NLM
STAT- MEDLINE
DCOM- 19990518
LR  - 20240109
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 11
IP  - 3
DP  - 1999 Mar
TI  - Discrete domains mediate the light-responsive nuclear and cytoplasmic 
      localization of Arabidopsis COP1.
PG  - 349-64
AB  - The Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) protein plays a critical 
      role in the repression of photomorphogenesis during Arabidopsis seedling 
      development. We investigated the control of COP1 partitioning between nucleus and 
      cytoplasm, which has been implicated in the regulation of COP1 activity, by using 
      fusion proteins between COP1 and beta-glucuronidase or the green fluorescent 
      protein. Transient expression assays using onion epidermal cells and data from 
      hypocotyl cells of stably transformed Arabidopsis demonstrated that COP1 carries 
      a single, bipartite nuclear localization signal that functions independently of 
      light. Nuclear exclusion was mediated by a novel and distinct signal, bordering 
      the zinc-finger and coiled-coil motifs, that was able to redirect a heterologous 
      nuclear protein to the cytoplasm. The cytoplasmic localization signal functioned 
      in a light-independent manner. Light regulation of nuclear localization was 
      reconstituted by combining the individual domains containing the nuclear 
      localization signal and the cytoplasmic localization signal; the WD-40 repeat 
      domain of COP1 was not required. However, phenotypic analysis of transgenic 
      seedlings suggested that the constitutively nuclear-localized WD-40 repeat domain 
      was able to mimic aspects of COP1 function, as indicated by exaggerated hypocotyl 
      elongation under light conditions.
FAU - Stacey, M G
AU  - Stacey MG
AD  - Department of Botany, University of Tennessee, HBB 437, Knoxville, Tennessee 
      37996-1100, USA.
FAU - Hicks, S N
AU  - Hicks SN
FAU - von Arnim, A G
AU  - von Arnim AG
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Nuclear Localization Signals)
RN  - 0 (Plant Proteins)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Arabidopsis/*chemistry
MH  - *Arabidopsis Proteins
MH  - Carrier Proteins/*chemistry/genetics
MH  - Cell Nucleus/chemistry
MH  - Cytoplasm/chemistry
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - *Nuclear Localization Signals
MH  - Plant Proteins/*chemistry/genetics
MH  - Structure-Activity Relationship
MH  - *Ubiquitin-Protein Ligases
PMC - PMC144184
EDAT- 1999/03/11 00:00
MHDA- 1999/03/11 00:01
PMCR- 2000/03/01
CRDT- 1999/03/11 00:00
PHST- 1999/03/11 00:00 [pubmed]
PHST- 1999/03/11 00:01 [medline]
PHST- 1999/03/11 00:00 [entrez]
PHST- 2000/03/01 00:00 [pmc-release]
AID - 10.1105/tpc.11.3.349 [doi]
PST - ppublish
SO  - Plant Cell. 1999 Mar;11(3):349-64. doi: 10.1105/tpc.11.3.349.