PMID- 10072485
OWN - NLM
STAT- MEDLINE
DCOM- 19990414
LR  - 20071115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 162
IP  - 5
DP  - 1999 Mar 1
TI  - Cutting edge: secondary lymphoid-tissue chemokine (SLC) and CC chemokine receptor 
      7 (CCR7) participate in the emigration pathway of mature dendritic cells from the 
      skin to regional lymph nodes.
PG  - 2472-5
AB  - Dendritic cells (DCs) emigrate to regional lymph nodes (LNs) during immune 
      responses via afferent lymphatic channels. Secondary lymphoid-tissue chemokine 
      (SLC), a CC chemokine, is expressed in secondary lymphoid organs and mediates the 
      chemotaxis of lymphocytes and DCs via its receptor, CC chemokine receptor 7 
      (CCR7). By dual-label fluorescence confocal microscopy, we showed MHC class 
      II-positive cells within SLC-staining lymphatic channels in the mouse dermis. SLC 
      was a potent in vitro chemoattractant for cultured, migratory skin DCs, and it 
      enhanced the emigration of MHC class II-positive DCs from mouse skin explants by 
      an average of 2.5-fold. Mature or cytokine-activated, but not resting, Langerhans 
      cells expressed CCR7 mRNA by RT-PCR. Anti-SLC Abs, but not control or 
      anti-eotaxin Abs, blocked the in vivo migration of 51Cr-labeled, skin-derived DCs 
      from footpads to draining LNs by 50% (n = 9, p < 0. 005). Thus, we provide direct 
      evidence that SLC and CCR7 participate in the emigration of DCs from peripheral 
      tissue to LNs via lymphatics.
FAU - Saeki, H
AU  - Saeki H
AD  - Branches ofDermatology and Experimental Immunology, National Cancer Institute, 
      Bethesda, MD 20892, USA.
FAU - Moore, A M
AU  - Moore AM
FAU - Brown, M J
AU  - Brown MJ
FAU - Hwang, S T
AU  - Hwang ST
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Ccl21c protein, mouse)
RN  - 0 (Ccr7 protein, mouse)
RN  - 0 (Chemokine CCL21)
RN  - 0 (Chemokines, CC)
RN  - 0 (Receptors, CCR7)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - Chemokine CCL21
MH  - Chemokines, CC/*physiology
MH  - Dendritic Cells/*physiology
MH  - Female
MH  - Lymph Nodes/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Receptors, CCR7
MH  - Receptors, Chemokine/*physiology
MH  - Skin/*immunology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1999/03/11 00:00
MHDA- 1999/03/11 00:01
CRDT- 1999/03/11 00:00
PHST- 1999/03/11 00:00 [pubmed]
PHST- 1999/03/11 00:01 [medline]
PHST- 1999/03/11 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1999 Mar 1;162(5):2472-5.