PMID- 10074507
OWN - NLM
STAT- MEDLINE
DCOM- 19990419
LR  - 20200724
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 37
IP  - 4
DP  - 1999 Apr
TI  - Amplification of the six major human herpesviruses from cerebrospinal fluid by a 
      single PCR.
PG  - 950-3
AB  - We used a novel type of primer system, a system that uses stair primers, in which 
      the primer sequences are based on consensus sequences in the DNA polymerase gene 
      of herpesvirus to detect herpesviruses by PCR. A single PCR in a single tube 
      detected the six major herpesviruses that infect the central nervous system: 
      herpes simplex virus type 1 (HSV-1), and type 2 (HSV-2), cytomegalovirus (CMV), 
      Epstein-Barr virus (EBV), varicella-zoster virus (VZV), and human herpesvirus 6 
      (HHV-6). We used the technique to analyze 142 cerebrospinal fluid (CSF) samples 
      that had been stored at -80 degrees C and compared the results with those 
      obtained previously for the same samples by standard, targeted PCR. Four hundred 
      one targeted PCR tests had been run with the 142 samples to detect HSV-1, HSV-2, 
      CMV, and VZV; screening for EBV and HHV-6 was not prescribed when the samples 
      were initially taken. Eighteen CSF samples tested positive by classic targeted 
      PCR. The herpesvirus consensus PCR detected herpesviruses in 37 samples, 
      including 3 samples with coinfections and 17 viral isolates which were not 
      targeted. Two samples identified as infected by the targeted PCR tested negative 
      by the consensus PCR, and eight samples that tested positive by the consensus PCR 
      were negative by the targeted PCR. One hundred three samples scored negative by 
      both the targeted and the consensus PCRs. This preliminary study demonstrates the 
      value of testing for six different herpesviruses simultaneously by a sensitive 
      and straightforward technique rather than screening only for those viruses that 
      are causing infections as suggested by clinical signs.
FAU - Minjolle, S
AU  - Minjolle S
AD  - Laboratoire de Bacteriologie-Virologie, CHU Pontchaillou, Rennes, France.
FAU - Michelet, C
AU  - Michelet C
FAU - Jusselin, I
AU  - Jusselin I
FAU - Joannes, M
AU  - Joannes M
FAU - Cartier, F
AU  - Cartier F
FAU - Colimon, R
AU  - Colimon R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Consensus Sequence
MH  - Cytomegalovirus/genetics/isolation & purification
MH  - DNA, Viral/*cerebrospinal fluid/*genetics
MH  - Diagnostic Errors
MH  - Evaluation Studies as Topic
MH  - Herpesviridae/classification/*genetics/*isolation & purification
MH  - Herpesviridae Infections/cerebrospinal fluid/diagnosis/virology
MH  - Herpesvirus 1, Human/genetics/isolation & purification
MH  - Herpesvirus 2, Human/genetics/isolation & purification
MH  - Herpesvirus 3, Human/genetics/isolation & purification
MH  - Herpesvirus 4, Human/genetics/isolation & purification
MH  - Herpesvirus 6, Human/genetics/isolation & purification
MH  - Humans
MH  - Polymerase Chain Reaction/*methods/statistics & numerical data
MH  - Sensitivity and Specificity
PMC - PMC88630
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
PMCR- 1999/04/01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
PHST- 1999/04/01 00:00 [pmc-release]
AID - 0911 [pii]
AID - 10.1128/JCM.37.4.950-953.1999 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1999 Apr;37(4):950-3. doi: 10.1128/JCM.37.4.950-953.1999.