PMID- 10074906
OWN - NLM
STAT- MEDLINE
DCOM- 19990521
LR  - 20131121
IS  - 1044-9523 (Print)
IS  - 1044-9523 (Linking)
VI  - 10
IP  - 2
DP  - 1999 Feb
TI  - Met-HGF/SF mediates growth arrest and differentiation in T47D breast cancer 
      cells.
PG  - 131-40
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a pluripotent growth factor 
      that exerts mitogenic, motogenic, and morphogenic effects. To elucidate the 
      cellular mechanisms underlying the pluripotent function of this growth factor, 
      T47D human breast cancer cells were transfected with human hgf/sf. The 
      hgf/sf-positive clones exhibited different levels of biologically functional 
      HGF/SF expression and up-regulation of endogenous Met (HGF/SF receptor) 
      expression. In addition, a constitutive phosphorylation of the receptor on 
      tyrosine residues was detected, establishing a Met-HGF/SF autocrine loop. The 
      autocrine activation of Met caused marked inhibition in cell growth accompanied 
      by cell accumulation at G0/G1. These cells underwent terminal cell 
      differentiation as determined by morphological changes, synthesis of milk 
      proteins such as beta-casein and alpha-lactalbumin, and production of lipid 
      vesicles. Our results demonstrate that Met-HGF/SF, an oncogenic signal 
      transduction pathway, is capable of inducing growth arrest and differentiation in 
      certain breast cancer cells and, thus, may have potential as therapeutic and/or 
      prognostic tools in breast cancer treatment.
FAU - Ronen, D
AU  - Ronen D
AD  - Department of Human Microbiology, Sackler School of Medicine, Tel Aviv 
      University, Israel.
FAU - Altstock, R T
AU  - Altstock RT
FAU - Firon, M
AU  - Firon M
FAU - Mittelman, L
AU  - Mittelman L
FAU - Sobe, T
AU  - Sobe T
FAU - Resau, J H
AU  - Resau JH
FAU - Vande Woude, G F
AU  - Vande Woude GF
FAU - Tsarfaty, I
AU  - Tsarfaty I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cell Growth Differ
JT  - Cell growth & differentiation : the molecular biology journal of the American 
      Association for Cancer Research
JID - 9100024
RN  - 0 (Caseins)
RN  - 42HK56048U (Tyrosine)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-49-2 (DNA)
RN  - 9013-90-5 (Lactalbumin)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Blotting, Western
MH  - Breast Neoplasms/*pathology
MH  - Caseins/metabolism
MH  - Cell Cycle/physiology
MH  - Cell Differentiation
MH  - Cell Division
MH  - DNA/metabolism
MH  - Flow Cytometry
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lactalbumin/metabolism
MH  - Methionine/metabolism
MH  - Microscopy, Fluorescence
MH  - Phenotype
MH  - Signal Transduction
MH  - Time Factors
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - Tyrosine/metabolism
MH  - Up-Regulation
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
PST - ppublish
SO  - Cell Growth Differ. 1999 Feb;10(2):131-40.