PMID- 10075022
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 49
IP  - 2
DP  - 1999 Feb
TI  - Phenotypic and functional properties of dendritic cells isolated from human 
      peripheral blood in comparison with mononuclear cells and T cells.
PG  - 177-83
AB  - Dendritic cells (DCs) are pivotal for antigen presentation, T-cell priming and 
      B-cell functions. Few studies have been carried out on DCs in human diseases, 
      partly because the current procedures used for DC preparation include elaborate 
      negative selection with monoclonal antibodies (MoAb) and prolonged culture in 
      cytokine-enriched milieu, which may influence DC functions. Using physical 
      density and their adherent properties, DCs were prepared from the blood of 
      healthy subjects. Approximately 2% of human blood mononuclear cells (MNC) were 
      shown to consist of DCs, yielding DCs of 80-90% purity. They expressed markers 
      related to DCs (CD1a, CD11c, CD32 and CD83), costimulatory molecules (CD40, CD80, 
      CD86), human leucocyte antigen (HLA) class I and II molecules and inducible 
      nitric oxide (NO) synthase (NOS2), and lacked lymphocyte and monocyte markers 
      (CD3, CD19, CD20, CD56 and CD14). Compared with blood MNC and T cells, DCs showed 
      a high level of spontaneous proliferation and nitric oxide production, as well as 
      strong proliferative responses in mixed leucocyte reactions. Enzyme-linked 
      immunospot (ELISPOT) assays revealed higher levels of interleukin (IL)-4-, IL-10- 
      and interferon-gamma (IFN-gamma)-secreting cells among DCs than among MNC or T 
      cells obtained from the same blood specimens, while levels of tumour necrosis 
      factor-alpha (TNF-alpha)- and IL-6-secreting cells did not differ. The results 
      demonstrate that the method used is fast, effective and competitively priced, and 
      should be useful for studies of DCs in disease states.
FAU - Huang, Y M
AU  - Huang YM
AD  - Department of Neurology, Karolinska Institute, Huddinge University Hospital, 
      Stockholm, Sweden.
FAU - Xiao, B G
AU  - Xiao BG
FAU - Westerlund, I
AU  - Westerlund I
FAU - Link, H
AU  - Link H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Antigens, CD)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, CD/analysis
MH  - Cell Division
MH  - Cell Membrane/immunology
MH  - Cell Separation/methods
MH  - Dendritic Cells/cytology/*immunology
MH  - Flow Cytometry
MH  - Humans
MH  - *Immunophenotyping
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-4/metabolism
MH  - Intracellular Fluid/immunology
MH  - Leukocyte Count
MH  - Leukocytes, Mononuclear/cytology/*immunology
MH  - Lymphocyte Activation
MH  - Lymphocyte Culture Test, Mixed
MH  - Nitric Oxide/biosynthesis
MH  - T-Lymphocytes/*immunology
EDAT- 1999/03/13 00:00
MHDA- 1999/03/13 00:01
CRDT- 1999/03/13 00:00
PHST- 1999/03/13 00:00 [pubmed]
PHST- 1999/03/13 00:01 [medline]
PHST- 1999/03/13 00:00 [entrez]
AID - 10.1046/j.1365-3083.1999.00491.x [doi]
PST - ppublish
SO  - Scand J Immunol. 1999 Feb;49(2):177-83. doi: 10.1046/j.1365-3083.1999.00491.x.