PMID- 10075443
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20190905
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 403
IP  - 1
DP  - 1999 Jan 5
TI  - Distribution of acetylcholinesterase and choline acetyltransferase in the main 
      and accessory olfactory bulbs of the hedgehog (Erinaceus europaeus).
PG  - 53-67
AB  - The distribution of cholinergic markers was studied in the main olfactory bulb 
      (MOB) and accessory olfactory bulb (AOB) of the western European hedgehog 
      (Erinaceus europaeus) by using choline acetyltransferase (ChAT) 
      immunocytochemistry and acetylcholinesterase (AChE) histochemistry. A dense 
      network of AChE-containing and ChAT-immunoreactive fibers was observed 
      innervating all layers of the MOB except the olfactory nerve layer, where neither 
      AChE- nor ChAT-labeled elements were found. The highest density of AChE- and 
      ChAT-positive axons was found in the glomerular layer (GL)/external plexiform 
      layer (EPL) boundary, and in the internal plexiform layer. This general 
      distribution pattern of ChAT- and AChE-stained axons resembled the distribution 
      pattern found in rodents. Nevertheless, some interspecies differences, such as 
      the lack of atypical glomeruli in the hedgehog, were also found. In addition to 
      fibers, a population of noncholinergic and presumably cholinoceptive AChE-active 
      neurons was observed in the hedgehog. All mitral and tufted cells of the hedgehog 
      MOB showed a dark AChE staining unlike previous observations in the mitral and 
      tufted cells of rodents. As in other species previously reported, subpopulations 
      of external tufted cells and short-axon cells were also AChE-active. Finally, a 
      population of small AChE-containing cells was observed in the EPL of the hedgehog 
      MOB. The size, shape, and location of these cells coincided with those of 
      satellite and perinidal cells, two neuronal types described previously in the EPL 
      of the hedgehog and not present in the rodent MOB. The AOB of the hedgehog showed 
      a distribution of AChE- and ChAT-positive fibers similar to the rodent AOB. 
      Nevertheless, a heterogeneous innervation of vomeronasal glomeruli by bundles of 
      AChE- and ChAT-labeled axons found in the hedgehog has not been previously found 
      in any other species. As in the MOB, all mitral cells in the AOB showed a strong 
      AChE activity. These results demonstrate some similarities but also important 
      differences between the distribution of ChAT and AChE in the MOB and AOB of 
      rodents and this primitive mammalian. These variations may indicate a different 
      organization of the cholinergic modulation of the olfactory information in the 
      insectivores.
FAU - Crespo, C
AU  - Crespo C
AD  - Departamento Biologia Celular y Patologia, Universidad de Salamanca, Spain.
FAU - Brinon, J G
AU  - Brinon JG
FAU - Porteros, A
AU  - Porteros A
FAU - Arevalo, R
AU  - Arevalo R
FAU - Rico, B
AU  - Rico B
FAU - Aijon, J
AU  - Aijon J
FAU - Alonso, J R
AU  - Alonso JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
SB  - IM
MH  - Acetylcholinesterase/*metabolism
MH  - Animals
MH  - Choline O-Acetyltransferase/*metabolism
MH  - Female
MH  - Hedgehogs/*metabolism
MH  - Histocytochemistry
MH  - Immunohistochemistry
MH  - Olfactory Bulb/*enzymology
MH  - Tissue Distribution
EDAT- 1999/03/13 03:11
MHDA- 2000/06/20 09:00
CRDT- 1999/03/13 03:11
PHST- 1999/03/13 03:11 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1999/03/13 03:11 [entrez]
AID - 10.1002/(SICI)1096-9861(19990105)403:1<53::AID-CNE5>3.0.CO;2-O [pii]
AID - 10.1002/(sici)1096-9861(19990105)403:1<53::aid-cne5>3.0.co;2-o [doi]
PST - ppublish
SO  - J Comp Neurol. 1999 Jan 5;403(1):53-67. doi: 
      10.1002/(sici)1096-9861(19990105)403:1<53::aid-cne5>3.0.co;2-o.