PMID- 10076513
OWN - NLM
STAT- MEDLINE
DCOM- 19990331
LR  - 20190815
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 144
IP  - 1
DP  - 1999
TI  - T cell responses to the structural polypeptides of porcine reproductive and 
      respiratory syndrome virus.
PG  - 117-34
AB  - The identification of antigens recognized by T cell responses has become 
      fundamental for developing effective immunizations against viral infections. 
      Lymphocyte proliferation and delayed-type hypersensitivity responses to porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection have been 
      demonstrated. However, the polypeptide specificity of T cell responses to PRRSV 
      is unknown. To identify the PRRSV polypeptides recognized by porcine lymphocytes 
      two approaches were employed. First polypeptides of purified virions were 
      separated by SDS-PAGE and particle suspensions obtained from nitrocellulose blots 
      were used as antigens. Second, the polypeptides encoded by ORFs 2, 4, 5, 6, and 7 
      of the strain VR-2332 were expressed as fusion proteins with a histidine tag in 
      mammalian cells, using vaccinia virus as expression system. Significant 
      antigen-specific proliferation responses to the matrix and envelope proteins from 
      purified virions were obtained. This finding was supported by specific and 
      dose-dependent proliferation responses to the recombinant polypeptides encoded by 
      ORF2, 5 and 6 detected in virus-infected but not in control pigs. These results 
      demonstrate that T-cell responses can be detected to individual PRRSV 
      polypeptides. The greater response to the product of ORF6 than to the other PRRSV 
      polypeptides indicates that the viral matrix polypeptide may have a major role in 
      cellular immunity.
FAU - Bautista, E M
AU  - Bautista EM
AD  - Department of Clinical and Population Sciences, College of Veterinary Medicine, 
      University of Minnesota, St. Paul, USA.
FAU - Suarez, P
AU  - Suarez P
FAU - Molitor, T W
AU  - Molitor TW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/*immunology
MH  - COS Cells
MH  - Cell Division
MH  - Humans
MH  - *Lymphocyte Activation
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - T-Lymphocytes/cytology/*immunology/*virology
MH  - Viral Structural Proteins/*immunology
EDAT- 1999/03/17 00:00
MHDA- 1999/03/17 00:01
CRDT- 1999/03/17 00:00
PHST- 1999/03/17 00:00 [pubmed]
PHST- 1999/03/17 00:01 [medline]
PHST- 1999/03/17 00:00 [entrez]
AID - 10.1007/s007050050489 [doi]
PST - ppublish
SO  - Arch Virol. 1999;144(1):117-34. doi: 10.1007/s007050050489.