PMID- 10079142
OWN - NLM
STAT- MEDLINE
DCOM- 19990427
LR  - 20061115
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 68
IP  - 3
DP  - 1999 Mar
TI  - Analysis of sex-mismatched human corneal transplants by fluorescence in situ 
      hybridization of the sex-chromosomes.
PG  - 341-6
AB  - The fate of the cells of corneal transplants has been controversial from the 
      early days of keratoplasty. Various methods such as histological evaluation, 
      radiolabeling of donor cells or Barr-body analysis have been applied to clarify 
      the issue. However, the question whether the transplanted cells are replaced or 
      survive, remains unsolved. In this study, we applied fluorescence in situ 
      hybridization (FISH) of the X- and Y-chromosomes in paraffin sections of 
      explanted sex-mismatched corneal transplants to distinguish between host and 
      donor cells. Fourteen sex-mismatched cases with various reasons for explantation 
      and different postoperative time intervals ranging from 11 months to 30 years 
      were analysed. We found that all cell types, including epithelium, keratocytes 
      and endothelial donor cells were replaced in most cases as early as 1 year after 
      transplantation. In three cases, however, up to 26% of donor keratocytes were 
      still detected up to 4.5 years after transplantation, demonstrating a certain 
      individual variability in the process of replacement. Further studies must show 
      if the extent and timing of donor cell replacement in clinically successful, 
      totally clear transplants is different. Our results are in keeping with the 
      phenomenon of recurrences of corneal dystrophies in the graft, the significant 
      postoperative decline of the endothelial cell density, the fact that typical 
      graft rejections usually take place within 1-2 years postoperatively and that 
      relatively late rejections can occur in rare cases probably due to some surviving 
      stromal keratocytes. Donor cell replacement is a special feature of corneal 
      transplants when compared with other kinds of organ transplants and might be due 
      to the presence of the same tissue type in the immediate neighbourhood of the 
      graft.
CI  - Copyright 1999 Academic Press.
FAU - Wollensak, G
AU  - Wollensak G
AD  - Eye Pathology Lab, Wilmer Eye Institute, Johns Hopkins Hospital, Baltimore, MD 
      21287-9248, USA.
FAU - Green, W R
AU  - Green WR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
SB  - IM
MH  - Cornea/*pathology
MH  - *Corneal Transplantation
MH  - Female
MH  - *Graft Rejection
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microscopy, Fluorescence
MH  - *Sex Chromosomes
MH  - Time Factors
MH  - Tissue Preservation
EDAT- 1999/03/18 00:00
MHDA- 1999/03/18 00:01
CRDT- 1999/03/18 00:00
PHST- 1999/03/18 00:00 [pubmed]
PHST- 1999/03/18 00:01 [medline]
PHST- 1999/03/18 00:00 [entrez]
AID - S0014-4835(98)90611-X [pii]
AID - 10.1006/exer.1998.0611 [doi]
PST - ppublish
SO  - Exp Eye Res. 1999 Mar;68(3):341-6. doi: 10.1006/exer.1998.0611.